枸杞多糖对Ⅱ型糖尿病小鼠胰岛细胞形态与功能的影响

采用高糖高热量饮食并结合小剂量腹腔注射STZ诱导Ⅱ型糖尿病（NIDDM）小鼠模型,枸杞多糖灌胃4周,用免疫组化技术对胰岛A、B细胞进行形态学分析及观察血糖、血清胰岛素的变化。结果显示,枸杞多糖大,小剂量组和模型组比较空腹血糖下降,胰岛素水平升高。图像分析大剂量组和模型组比较,B细胞数密度增加、A细胞数密度减小、B细胞切面积、核面积、核质比升高。结论：枸杞多糖可以改善Ⅱ型糖尿病小鼠胰岛细胞形态和功能并促进胰岛素的分泌,有良好的降血糖作用。

Effect of lycium barbarum polysaccharide on morphogeny and function of pancreatic islet B cells in NIDDM mice

The non-insulin dependent diabetes mellitus (NIDDM) mice induced by high-sugar,high-fat diet and injecting little dose of STZ were treated with LBP (20mg/kg,40mg/kg) for 4 weeks.The effects of LBP were then evaluated by examining fasting blood glucose (FBG),blood insulin and analysising A,B cells of pancreatic islet by immunohistochemistry technology. The results displayed that the level of FBG is lower in the groups of LBP (including the dose of high and low) than that in model group and the level of insulin is increased.The density of B cells is increasing,but A cells is decreasing and the section area,nuclear area,nuclear-to-cytoplasm ratio of B cells are higher in LBP group treated with high dose than those in model group.In conclusion, LBP possess the action of lowing blood glucose,improving morphogeny and function of pancreatic islet B cells and increasing the secretion of insulin in NIDDM mice.