藏红花球茎组织培养条件的研究

以藏红花球茎侧芽为外植体,通过植物组织培养的方式建立藏红花快速繁殖体系。研究表明:以MS为基本培养基,附加0.5 mg·L^-16-BA+4.0 mg·L^-12,4-D,在黑暗条件下最适于藏红花愈伤组织的诱导,并且20 d诱导率可以达到96.7 %;附加2.0 mg·L^-16-BA+0.5 mg·L^-1NAA,在黑暗条件下最适合藏红花丛生芽的诱导与增值,丛生芽诱导率可达96 %;附加5.0 mg·L^-16-BA +1.5 mg·L^-1NAA+0.3 g·L^-1AC,在1 500～2 000 lx的光照条件下,30 d左右新生小球茎诱导率高达90 %。

Study on Tissue Culture Conditions of Saffron Corm

A rapid propagation system of saffron (Crocus sativus L.) was established here using corms of saffron as explants by means of plant tissue culture. When the corms of saffron were cultured in the dark on MS medium containing 0.5 mg/L 6-benzyladenine (6-BA) and 4.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days, the callus induction rate was as high as 96.7%. When the corms of saffron were cultured in the dark on MS medium containing 2.0 mg/L 6-benzyladenine (6-BA) and 0.5 mg/L naphthalene acetic acid (NAA) for 20 days, the callus induction rate was as high as 96%. When the corms of saffron were cultured in 1500 ~ 2000 lx lighting condition on MS medium containing 5.0 mg/L 6-benzyladenine (6-BA), 1.5 mg/L naphthalene acetic acid (NAA) and 0.3 g/L activated carbon (AC) for about 30 days, the cormels induction rate was as high as 90%.