PCR技术在基因突变检测中的应用

ＰＣＲ（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ）技术以其具有高特异性、高效率、高灵敏度而广泛地应用于基因突变研究中，近年来，ＰＣＲ技术不仅推动了以往基因突变检测技术的发展，而且建立于ＰＣＲ技术之上的新技术设计先进、操作方便省时，具有更广阔的实用性。文中对这些基因突变检测技术的原理、特点及应用给以综述。

The Application of PCR Technique in the Check Up of Gene Mutation

the palymerase chain reaction(PCR)is widely applied in the research of gene mutation, for its high efficiency, specification and sensitivity. In recent years, not only PCR propel development of former check up technique of gene mutation,but also the latest techmque based on PCR come into being with advanced design, easy operation and wide feasibility. has been summarized in this paper The principle, features and application of check up technique of gene mutation.