| 川芎道地性的ISSR分析 |
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| 引用本文： | 王岚,肖海波,马逾英,蒋桂华,唐琳. 川芎道地性的ISSR分析[J]. 四川大学学报(自然科学版), 2008, 45(6): 1472-1476 |
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| 作者姓名： | 王岚 肖海波 马逾英 蒋桂华 唐琳 |
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| 作者单位： | 1. 四川大学生命科学学院,成都,610064
2. 成都中医药大学药学院,成都,610075 |
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| 基金项目： | “十五”国家科技攻关课题 ( 2004BA721A31) |
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| 摘 要： | 利用ISSR分子标记技术,对来自国内17个居群的285个川芎(Ligusticum chuanxiong hort.)个体进行道地性分析.从99条ISSR随机引物中筛选出10条引物对285个川芎个体进行扩增,共得到186条清晰的扩增位点,其中多态性位点183个,多态位点百分率（PPB）为98.39％.根据结果进行聚类分析,得出16个川芎居群遗传距离的非加权算术平均(UPGMA)树.采自四川省内的10个川芎居群表现出更近的亲缘关系,各个川芎居群间具有高度遗传多样性.
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| 关 键 词： | 道地性 川芎 |
Analysis on genuineness of Ligusticum chuanxiong hort.by ISSR |
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| WANG Lan,XIAO Hai-Bo,MA Yu-Ying,JIANG Gui-Hua,TANG Lin. Analysis on genuineness of Ligusticum chuanxiong hort.by ISSR[J]. Journal of Sichuan University (Natural Science Edition), 2008, 45(6): 1472-1476 |
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| Authors: | WANG Lan XIAO Hai-Bo MA Yu-Ying JIANG Gui-Hua TANG Lin |
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| Affiliation: | College of Life Sciences, Sichuan University;College of Life Sciences, Sichuan University;College of Pharmacology, Chengdu University of TCM;College of Pharmacology, Chengdu University of TCM;College of Life Sciences, Sichuan University |
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| Abstract: | Inter-simple sequence repeats(ISSR) markers were used to investigate the genuineness of 285 individuals from 17 L.chuanxiong populations in China.Based on 10 primers,186 ISSR bands were generated,183(98.39%) were polymorphic.The 17 populations of L.chuanxiong were clustered in unweighted pair group method with arithmetic average(UPGMA) tree.10 populations from Sichuan Province clustered together before forming a cluster with other populations elsewhere,illustrated that they have nearer relationships.A high ... |
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| Keywords: | ISSR |
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