异丙肾上腺素致大鼠心肌缺血型慢性心力衰竭的实验研究

目的:建立异丙肾上腺素(ISO)缺血型慢性心力衰竭(CHF)大鼠模型,为研究慢性心衰的发病机制和有效治疗措施提供理想的动物模型。方法:模型组大鼠首次按3.0 mg/(kg.d)腹腔注射ISO连续3 d,观察4周,第5周按2.0 mg/(kg.d)继续给药2 d,观察1周,第6周再按3.0 mg/(kg.d)给药2 d,1周后进行血流动力学指标、血清心肌肌钙蛋白-Ⅰ(cTn-Ⅰ)、血管紧张素Ⅱ(AngII)水平等测定和肺组织病理学观察。结果:模型组大鼠较正常对照组左室收缩最大速率降低25.2%,左室舒张最大速率绝对值降低了33.7%,肺组织病理改变明显,血清cTn-Ⅰ和AngII水平明显增加(P<0.01)。结论:采用大鼠间断性腹腔反复注射一定剂量ISO制备慢性心衰模型方法稳定、可靠、容易控制。

Experimental Study of Isoproterenol-induced Ischemic Chronic Heart Failure in the Rat

Objective:To establish a model of isoproterenol-induced ischemic CHF in the rat,and provide a useful animal model for studies on pathogenesis and effective therapy in CHF.Methods: The model group was injected intraperitoneally(ip) with ISO at 3.0 mg/(kg/d) for 3 consecutive d and then was observed for four weeks.In the fifth week,the model group was injected(ip) sequentially with ISO at 2.0 mg/(kg·d) for 2 consecutive d and then was observed for one week.In the sixth week,the model group was injected(ip) with ISO at 3.0 mg/(kg·d) for2 consecutive d.One week later,the hemodynamic,serum cTn-Ⅰ and Ang II were measured.Lung tissue pathological changes were observed.Results: Model groups rats showed that the value of +dp/dtmax reduced 33.7 percent,and the absolute value of-dp/dtmax reduced 25.2 percent.Lung tissue pathological changes were obvious.The serum cTn-Ⅰ and Ang II increased significantly(P<0.01).Conclusion: Model of CHF in the rats was induced by ISO multiple and discontinuity ip of at a certain dose and it is reliable and easy to copy and control.