| 缓冲液对SOD-邻苯三酚自氧化的影响 |
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| 引用本文： | 于秀芝,刘曼西,郑坚.缓冲液对SOD-邻苯三酚自氧化的影响[J].华中科技大学学报(自然科学版),1989(4). |
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| 作者姓名： | 于秀芝 刘曼西 郑坚 |
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| 作者单位： | 华中理工大学生物工程系
(于秀芝，刘曼西)，华中理工大学生物工程系(郑坚) |
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| 摘 要： | 超氧化物歧化酶(Superoxide dismutase,简称SOD)由于能消除对细胞极毒的O_i~-,在医学上颇受重视,正成为极有价值的临床指标和治疗酶。有关此酶的测定方法也得到不断改进。我们在测定某些药物对小白鼠器官SOD水平影响的实验中,比较了几种方法,认为邻苯三酚自氧化法较为适用,该法的关键是缓冲液的选择。选用了1/(15)M,pH7.0～8.2的磷酸氢二钠-磷酸二氢钾缓冲液(简称Na~+-K~+型PBS),获得了较宽的线性范围,重现性好。
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The Effect of Buffer on SOD and Auto-Oxidation of Pyrogallol |
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| Yu Xiuzhi Liu Manxi Zheng Jian.The Effect of Buffer on SOD and Auto-Oxidation of Pyrogallol[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,1989(4). |
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| Authors: | Yu Xiuzhi Liu Manxi Zheng Jian |
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| Institution: | Yu Xiuzhi Liu Manxi Zheng Jian |
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| Abstract: | Instead of traditionally used buffers, M/15 Na+-K+ phosphate buffer (pH) 7.0 -8.2 is used to determine SOD avidity by auto-oxidation of pyrogallol. The holding time on linearity is longer than 10 minutes and the repeatability is fine. |
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| Keywords: | Superoxide dismutase Buffer Pyrogallol Auto-oxidation |
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