| 荧光法研究3-环丙基-3-去乙烯基红紫素18甲酯、3-环丙基-3-去乙烯基-N-甲氧基红紫素18酰亚胺甲酯和转铁蛋白的相互作用 |
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| 引用本文： | 刘淑燕,;刘振波,;刘永明,;王进军.荧光法研究3-环丙基-3-去乙烯基红紫素18甲酯、3-环丙基-3-去乙烯基-N-甲氧基红紫素18酰亚胺甲酯和转铁蛋白的相互作用[J].烟台师范学院学报(自然科学版),2008(1). |
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| 作者姓名： | 刘淑燕 ;刘振波 ;刘永明 ;王进军 |
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| 作者单位： | [1]烟台大学药学院; [2]烟台大学化学院; [3]烟台大学药学院,山东烟台264005; [4]山东烟台264005,山东烟台264005; |
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| 摘 要： | 用荧光光谱法、紫外-可见分光光度法研究了3-环丙基-3-去乙烯基红紫素18甲酯(MRNB-2)、3-环丙基-3-去乙烯基-N-甲氧基红紫素18酰亚胺甲酯(MRNB-4)与转铁蛋白(Tf)的相互结合反应.实验表明,MRNB-2,MRNB-4与Tf的相互结合作用为单一的静态猝灭过程,在溶液中二者以1∶1的摩尔比牢固结合,25℃时结合反应的平衡常数分别为KMRNB-2=7.64×105L/mol,KMRNB-4=2.09×104L/mol.根据Frster非辐射能量转移机理,求算了给体(Tf)与受体(MRNB-2,MRNB-4)间距离(r)和能量转移效率(E)分别为rMRNB-2=3.85 nm,rMRNB-4=4.14 nm;EMRNB-2=0.340,EMRNB-4=0.127.并推测二者之间的主要作用力为疏水作用力.
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| 关 键 词： | 光动力疗法 转铁蛋白 荧光猝灭 结合反应 |
Study on the Interaction Between MRNB-2,MRNB-4 and Transferrin |
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| Institution: | LIU Shu-yan1; LIU Zhen-bo2; LIU Yong-ming2; WANG Jin-jun1; 2 (1.Shool of Pharmacy; 2.College of Chemistry; Yantai University; Yantai 264005; China); |
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| Abstract: | The binding reaction between MRNB-2,MRNB-4 and transferring(Tf) was studied by fluorescence and UV-Vis absorption spectra. The research results indicate that the combination reaction of them is a single static quenching process,and MRNB-2,MRNB-4 strongly bound Tf with the molar ratio of 1∶1 and the binding equilibrium constant K0 at 25℃,KMRNB-2=7.64×105 L/mol,KMRNB-4=2.09×104 L/mol.The shortest binding distance r and energy transfer efficiencies E between donor Tf and acceptor of MRNB-2,MRNB-4 are obtained ... |
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| Keywords: | photodynamic therapy transferring fluorescence quenching binding reaction |
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