| 基于金纳米棒基因探针对多元基因的识别与检测 |
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| 引用本文： | 孙桂敏,刘媚,杨培慧,蔡继业.基于金纳米棒基因探针对多元基因的识别与检测[J].科学通报,2011,56(13):1007-1012. |
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| 作者姓名： | 孙桂敏 刘媚 杨培慧 蔡继业 |
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| 作者单位： | 暨南大学化学系;光电信息与传感技术广东普通高校重点实验室; |
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| 基金项目： | 国家自然科学基金(21071064); 国家重点基础研究发展计划(2010CB833603)资助项目 |
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| 摘 要： | 通过晶种生长法制备长径比分别为4:1 和8:1 的金纳米棒, 分别在其表面修饰上抑癌基因p53 和pTEN 2 种不同序列的模型基因片段, 构建金纳米棒基因探针, 这两种基因探针分别于800 和1100 nm 处有2 个近红外吸收峰. 基于探针基因识别靶基因时, 金纳米棒的纵向等离子共振吸收峰吸光度的变化, 对2种靶基因片段混合体系建立特异性识别及检测方法. 结果表明, 在pH 为7.0 的PBS 缓冲溶液中, NaCl 浓度为0.2mol/L 时, 在靶基因片段的浓度为3.0~9.0 nmol/L 范围内, 金纳米棒的纵向等离子共振吸收峰的变化与靶基因的浓度成线性关系, 检出限分别为1.6 和1.2 nmol/L. 实现了在同一混合体系中同时对两种靶基因片段的特异性识别及检测.
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| 关 键 词： | 金纳米棒 表面等离子共振吸收 基因探针 |
| 收稿时间： | 2010-12-20 |
Simultaneous recognition and detection of multiplex genes by gold nanorod gene probes |
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| Sun GuiMin,Liu Mei,Yang PeiHui,Cai JiYe.Simultaneous recognition and detection of multiplex genes by gold nanorod gene probes[J].Chinese Science Bulletin,2011,56(13):1007-1012. |
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| Authors: | Sun GuiMin Liu Mei Yang PeiHui Cai JiYe |
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| Institution: | 1. Department of chemistry, Ji’nan University, Guangzhou 510632, China;
2. Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Guangzhou 510632, China |
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| Abstract: | Gold nanorods, with mean aspect ratios of 4 and 8, were synthesized by seed-mediated growth. Sequences of Antioncogene p53 and pTEN were used as model gene probes. Surface modification of gold nanorods with the different probe genes yielded gold nanorod gene probes. The Antioncogene p53 and pTEN gold nanorod gene probes both showed a weak transverse plasmon band at 520 nm and a strong longitudinal plasmon band at 800 and 1100 nm, respectively. Two different target gene sequences were detected, based on the ... |
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| Keywords: | gold nanorod surface plasmon resonance gene probe |
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