| 青杨组织培养快速繁殖 |
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| 引用本文： | 耿飒,姬生栋,袁金云,周春娥,赵晓进.青杨组织培养快速繁殖[J].河南师范大学学报(自然科学版),2006,34(2):103-105. |
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| 作者姓名： | 耿飒 姬生栋 袁金云 周春娥 赵晓进 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡453007 |
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| 基金项目： | 国家自然科学基金
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河南省重点学科建设项目 |
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| 摘 要： | 建立了青杨(Populus cathayana)组织培养系统.最适合的外植体是新切下的枝条插入含有蛭石和充足水的营养钵中24 h以上,使其在弱光下迅速长出嫩枝的茎切段和茎尖.茎切段和茎尖外植体经0.1%HgCl2或5%NaClO溶液表面消毒后,接种到大量元素减半、含0.5 mg/L 6-BA和0.03 mg/L NAA的MS培养基上诱导芽,试管苗的生根采用含0.5 mg/L IBA的MS培养基.试管苗茎段的分化依外源激素条件的不同而异.
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| 关 键 词： | 芽 青杨 组织培养 |
| 文章编号： | 1000-2367(2006)02-0103-03 |
| 收稿时间： | 2005-04-25 |
| 修稿时间： | 2005年4月25日 |
Rapid Regeneration of Cathay Poplar by Tissue Culture |
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| GENG Sa,JI Sheng-dong,YUAN Jin-yun,ZHOU Chun-e,ZHAO Xiao-jin.Rapid Regeneration of Cathay Poplar by Tissue Culture[J].Journal of Henan Normal University(Natural Science),2006,34(2):103-105. |
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| Authors: | GENG Sa JI Sheng-dong YUAN Jin-yun ZHOU Chun-e ZHAO Xiao-jin |
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| Institution: | College of Life Science, Henan Normal University, Xinxiang 453007,China |
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| Abstract: | The tissue culture system of Cathay Poplar(Populus cathayana) was developed in the present paper.The most suitable explants for in vitro growth were shoot-tips and stem segments from sprouting cutting which were plugged in water or in vermiculite under weak light more than 24 h.Surface sterilization of explants was accomplished by 0.1 % HgCl_2 or 5% NaClO.The most suitable medium for differentiation of stem segment from plantlets was the modified MS(half of macroelements) with 0.3 mg/L 6-BA and 0.03 mg/L NAA and MS with 0. 3 mg/L IBA for rooting in vitro.The differentiation of stem segment of plantlets depended on the concentration of exogenous hormone. |
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| Keywords: | shoot Populus cathayana tissue culture |
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