| DNA与抗癌药物道诺霉素相互作用的研究 |
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| 引用本文： | 赵昌后,孙星炎.DNA与抗癌药物道诺霉素相互作用的研究[J].华东师范大学学报(自然科学版),1999(3):68-71. |
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| 作者姓名： | 赵昌后 孙星炎 |
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| 作者单位： | 华东师范大学化学系!上海200062 |
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| 摘 要： | 该文采用荧光光谱法并用溴化乙锭（ＥＢ）作为荧光探针研究了道诺霉素（ＤＲＮ）与ＤＮＡ相互作用的方式。结果表明：ＤＲＮ的生色团能够嵌入ｄｓＤＮＡ双螺旋结构的碱基对之间，ＤＲＮ与ＤＮＡ的主要作用位点是碱基Ｇ、Ｃ；ＤＲＮ插入ｄｓＤＮＡ碱其对中，不会破坏碱基对中氢键，不会影响ＤＮＡ的复性；ＤＮＡ与ｓｓＤＮＡ仅仅存在弱的相互作用。
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| 关 键 词： | 相互作用 道诺霉素 DNA 抗癌药物 |
Study of the Interaction of DNA with Anticancer-drug DRN |
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| Zhao Changhou,Sun Xingyan,Liu Shenghui,Xu Chun,Fang Yuzhi.Study of the Interaction of DNA with Anticancer-drug DRN[J].Journal of East China Normal University(Natural Science),1999(3):68-71. |
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| Authors: | Zhao Changhou Sun Xingyan Liu Shenghui Xu Chun Fang Yuzhi |
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| Abstract: | The interacion of anticancer-drug daunomycin (DRN) with DNA was studied by fluorescence spectroscopy. The results demonstrated flrorescence quenching was significantly observed in the presence of dsDNA, but ssDNA had slightly effect on the daunomycin fluorescence. The results show daunomycin interaction with DNA by intercalation of the chromophore between base pairs. The main interacion sites are G and C. When DRN intercalates into DNA, it does not disrupt the hydrogen bonds between bases of DNA andeffect on hybridization of DNA. |
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| Keywords: | DRN DNA interaction fluorescence spectroscopy |
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