用大鼠心肌条件培养基建立来源于C57BL/6J小鼠的ES细胞系

报道一种新的建立Ｃ５７ＢＬ／６Ｊ小鼠ＥＳ细胞系的方法，采用大鼠心肌条件培养基，在不使用饲养层细胞和白血病抑制因子（ＬＩＦ）的情况下，从Ｃ５７ＢＬ／６Ｊ品系小鼠中建成１个ＥＳ细胞系即ＭＥＳＰＵ４１，成系率为１．０％。ＭＥＳＰＵ４１细胞为ＸＸ型，核型正常率高达８９％，表现出ＸＸ型ＥＳ细胞系少有的稳定性。进行体内分化实验时ＭＥＳＰＵ４１细胞能发生广泛分化形成畸胎瘤。嵌合体制作实验证实ＭＥＳＰＵ４１细胞具

Establishment of a C57BL/6J ES Cell Line by Conditioned Media of Rat Myocardial Cells

A new method of deriving ES cell lines from C57BL/6J mice was described. One C57BL/6J ES cell line (MESPU?41) was established in medium conditioned by rat myocardial cells, without the use of feeder cells and Leukemia Inhibitory Factor(LIF). The frequency of establishment was 1.0%. MESPU?41 cells was XX type. 89% of them showed karyotypically normal and stable which were rare in XX type ES cells. They can differentiate into wide types of cells and form teritocarcinoma in vivo. Chimeras were made by injecting the ES cells into blastocysts, indicating that they had the potency to participate into the development of embryos. Using RT\|PCR method, we found that rat myocardial cells expressed LIF mRNA, which might suppress ES cells differentiation and stabilize X chromosome. In order to simplify the process of preparing conditioned medium, we also immortalized rat myocardial cells. These methods broadened the conditions for the establishment of ES cell lines and open up a new point to study the stability of cultured ES cells.