| 荧光光度法测定大豆提取液中还原型谷胱甘肽 |
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| 引用本文： | 郭黎平,刘国良,张卓勇,刘思东,王丽颖. 荧光光度法测定大豆提取液中还原型谷胱甘肽[J]. 东北师大学报(自然科学版), 2001, 33(1): 34-38 |
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| 作者姓名： | 郭黎平 刘国良 张卓勇 刘思东 王丽颖 |
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| 作者单位： | 东北师范大学化学学院,;白求恩医科大学基础医学院, |
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| 基金项目： | 吉林省卫生厅科研基金资助项目 |
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| 摘 要： | 在Cohn的荧光分光光度法的基础上,不加去蛋白试剂,用365nm作为激光发波长,探讨了用荧光光度法测定大豆提取液中的还原型谷胱甘肽的方法,确定了最佳测定条件,该方法用于测定大豆提取液中谷胱甘肽的含量,取得了满意结果。
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| 关 键 词： | 还原型谷胱甘肽 大豆提取液 荧光光度法 |
| 文章编号： | 1000-1832(2001)01-0034-05 |
| 修稿时间： | 2000-09-26 |
Determination of the reduced glutathione by fluorescence spectrophotometry |
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| GUO Li-ping,LIU Guo-liang,ZHANG Zhuo-Yong,LIU Si-dong,WANG Li-ying. Determination of the reduced glutathione by fluorescence spectrophotometry[J]. Journal of Northeast Normal University (Natural Science Edition), 2001, 33(1): 34-38 |
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| Authors: | GUO Li-ping LIU Guo-liang ZHANG Zhuo-Yong LIU Si-dong WANG Li-ying |
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| Abstract: | An improved fluorescence spectrophotometric method was developed for the reduced glutathione(GSH) determination based on the reaction of GSH with orthophthaldehyde (OPA). Some affecting parameters including amount of OPA, stability of the fluorescence system, etc. were investigated and optimized. Linear dynamic relationship was obtained in the concentration range of 0.08～0.8 μg/mL for glutathione. The regression equation was as following: I=0.984 4+84.15c. T he correlation coefficient was 0.999 9. The method was also applied to determine GSH in soybean extracts by electromagnetic field technique and satisfactory results were obtained. |
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| Keywords: | reduced glutathione soybean extracts fluorescence spectrophotometry |
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