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Super-distant molecular hybridization of plant seeds by ion beam-mediated gene cluster
Authors:SONG Daojun  CHEN Ruolei  WU Lifang  WANG Cen  ZHANG Jian  YU Zengliang
Institution:1. Center of Fengle lon Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences,Hefei 23003l, China
2. Department of Life Science & Technology, Zhejiang University, Hangzhou 310027, China
3. Institute of Biological Machinery, Hefei Industrial University, Hefei 230069, China
Abstract:The N beam-mediated distant molecular hybridization between Ginkgo biloba I and watermelon was studied. The results showed that the ester gene of Ginkgo biloba L was successfully expressed in two varieties of watermelon. 3-16 and SR2-14-2, in both of which the ester quantities were measured as 17.0756 μg/g and 45.9998 μg/g respectively. Meanwhile, superoxide dismutase (SOD) activity in leaves of the watennelon expressing ester gene was increased twofold as compared to that of the control, showing that ion beam could mediate distant and/or super-distant donor gene expression in the cells of a receptor. Furthermore, the molecular nechanism of distant hybridization was analyzed.
Keywords:ion beam  distant hybridization  Ginkgo biloba L  watermelon  ester of ginkgo biloba L  superoxide dismutase
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