Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E |
| |
Authors: | T Hamada |
| |
Institution: | (1) Laboratory of Trace Elements Nutrition, National Institute of Animal Industry, Tsukuba Norindanchi POB 5, 305 Ibaraki, (Japan);(2) Present address: Department of Animal Science, Tokyo University of Agriculture, Sakuragaoka, 156 Setagaya, (Japan) |
| |
Abstract: | The concentration-dependent effects of copper acting either as an antioxidant or as a prooxidant were examined in vitro using Tween 20-induced hemolysis. When cupric ion concentration was more than 10 M, free copper(II) acted as a prooxidant; both extensive hemolysis and production of unknown thiobarbituric acid-reactive substance occurred in hamster and pig erythrocytes irrespective of vitamin E status. However, when cupric ion concentration was 2–4 M in the incubation medium, copper showed a clear antioxidant activity, reducing both hemolysis and malondialdehyde production induced either by diluted peroxide-containing Tween 20 with ascorbic acid and sodium azide in vitamin E-deficient hamster erythrocytes, or by peroxide-containing Tween 20 in pig erythrocytes containing marginal amounts of vitamin E. Copper(II) is taken up by the erythrocytes, where copper(I)-complexes may contribute to the protection of cells with membrane vitamin E against oxidative radical attack. |
| |
Keywords: | Copper vitamin E Tween 20 hemolysis malondialdehyde prooxidant antioxidant |
本文献已被 SpringerLink 等数据库收录! |
|