| 反相高效液相色谱法测定发酵液中子囊霉素含量 |
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| 引用本文： | 王瑶函,杨文革,胡永红.反相高效液相色谱法测定发酵液中子囊霉素含量[J].南京工业大学学报(自然科学版),2011,33(1):107-110. |
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| 作者姓名： | 王瑶函 杨文革 胡永红 |
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| 作者单位： | 南京工业大学,生物与制药工程学院,江苏,南京,210009 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，江苏省"六大人才高峰计划"，江苏省高校研究生科技创新计划 |
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| 摘 要： | 建立用反相高效液相色谱法(RP-HPLC)测定发酵液中子囊霉素含量方法。采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,以V(乙腈)∶V(水)=70∶30为流动相,柱温40℃,流速1.0 mL/min,检测波长210 nm,进样量20μL。子囊霉素标准品质量浓度在0.02~2.0 mg/mL范围内线性关系良好,线性回归方程为ρ=0.003 88A-0.001 41,r=0.999 97。平均回收率为99.79%(n=9),相对标准偏差(RSD)为0.45%。
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| 关 键 词： | RP-HPLC 子囊霉素 含量测定 |
Determination of ascomycin in fermentation broth by reversed-phase liquid chromatography |
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| WANG Yaohan,YANG Wenge,HU Yonghong.Determination of ascomycin in fermentation broth by reversed-phase liquid chromatography[J].Journal of Nanjing University of Technology,2011,33(1):107-110. |
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| Authors: | WANG Yaohan YANG Wenge HU Yonghong |
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| Institution: | (Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | Reversed-phase liquid chromatography(RP-HPLC) for the quantitative determination of ascomycin in the fermentation broth was established out on a Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was acetonitrile-water of 70∶30 with the flow rate of 1.0 mL/min,wave length of 210 nm and column temperature of 40 ℃.The results showed that the external standard calibration curve for quantify ascomycin by RP-HPLC was validated from 0.02 mg/mL to 2.0 mg/mL with a satisfied linear relationship.The average recovery rate of the ascomycin was 99.79%(n=9) with the relative standard deviation(RSD) of 0.45%. |
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| Keywords: | RP-HPLC |
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