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对虾白斑综合征病毒(WSSV)基因组细菌双杂交系统的构建
引用本文:熊生良,杨丰. 对虾白斑综合征病毒(WSSV)基因组细菌双杂交系统的构建[J]. 厦门大学学报(自然科学版), 2006, 45(5): 696-699
作者姓名:熊生良  杨丰
作者单位:1. 厦门大学生命科学学院,福建,厦门,361005
2. 国家海洋局第三海洋研究所,福建,厦门,361005
基金项目:国家自然科学基金,福建省自然科学基金
摘    要:细菌双杂交系统是新近建立的一种研究蛋白质间相互作用的方法.应用细菌双杂交系统,分别以pBT、pTRG诱饵质粒构建编码WSSV基因组DNA随机片段的融合表达质粒pBT-wssv、pTRG-wssv.重组质粒共转化双杂交报告菌株XLl-Blue MRF′,通过LB-TCK平板筛选对虾白斑综合征病毒中有相互作用的蛋白质.本研究中我们构建了WSSV基因组细菌双杂交系统,用于筛选WSSV中有相互作用的蛋白,为该病毒功能基因组的研究打下良好的基础.

关 键 词:对虾白斑综合征病毒  细菌双杂交  pBT诱饵质粒  pTRG诱饵质粒  共转化
文章编号:0438-0479(2006)05-0696-04
收稿时间:2006-03-24
修稿时间:2006-03-24

Construction of White Spot Syndrome Virus (WSSV) Whole Genome Bacterial Two-hybrid System Library
XIONG Sheng-liang,YANG Feng. Construction of White Spot Syndrome Virus (WSSV) Whole Genome Bacterial Two-hybrid System Library[J]. Journal of Xiamen University(Natural Science), 2006, 45(5): 696-699
Authors:XIONG Sheng-liang  YANG Feng
Affiliation:1. School of Life Sciences, Xiamen University, Xiamen 361005, China 2. The Third Institute of Oceanography,SOA,Xiamen 361005 ,China
Abstract:Bacterial two-hybrid system is a newly developed method for studying protein-protein interaction.We used BacterioMatch two-hybrid system(Stratagene) to study the interaction of WSSV protein.First,the random segments(about 1 kb) of WSSV genome were inserted into the plasmids pBT and pTRG to construct recombinant plasmids pBT-wssv and pTRG-wssv respectively.Then these two plasmids were cotransformed into the competent bacteria XL1-Blue MRF'.And a reporter gene had been embedded into this strain.Therefore,we used LB-TCK agar plate to screen the WSSV protein in order to find the interaction of them.In current research we had constructed the WSSV gene group bacterial two-hybrid system,which had been used to screen the WSSV genome to find the protein interaction.And we also built a good foundation for this viral functional genome research.
Keywords:white spot syndrome virus(WSSV)  bacterial Two-Hybrid  pBT bait plasmid  pTRG bait plasmid  cotransform
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