| 农杆菌介导的胆碱脱氢酶基因(betA)转化小麦及影响转化效率的因素 |
| |
| 引用本文： | 薛哲勇,贺晨霞,孙松,夏光敏.农杆菌介导的胆碱脱氢酶基因(betA)转化小麦及影响转化效率的因素[J].山东大学学报(理学版),2004,39(3):104-110,115. |
| |
| 作者姓名： | 薛哲勇 贺晨霞 孙松 夏光敏 |
| |
| 作者单位： | 山东大学,生命科学学院,山东,济南,250100 |
| |
| 基金项目： | 国家转基因专项资助项目(J99-037) |
| |
| 摘 要： | 用LBA4404／pCDH，Agl 1／pUNN2和Ag;P(无质粒)3种菌株分别转化小麦品种济南177、99P、核生3号幼胚诱导的愈伤组织以及济南177的幼胚．其中以胚性愈伤组织为外植体，获得了转基因植株．PCR和PCR-Southem分析证实转化植株中包含了外源基因．通过染色体分析，发现胚性愈伤组织在农杆菌LBA4404侵染后形成的染色体削减比经Agl1侵染和未经感染的对照形成的染色体削减程度更大，甚至发现较多的染色体断片．分析了农杆菌菌株，材料的基因型，外植体类型，培养时间和温度以及筛选的时间和周期对于转化效率的影响．
|
| 关 键 词： | 小麦 农杆菌转化 染色体消减和断裂 转基因植株 转化效率 |
| 文章编号： | 1671-9352(2004)03-0104-07 |
Agrobacterium tumefaciens mediated choline dehydrogenase gene(betA) transfer to wheat and factors impacting on transformation |
| |
| XUE Zhe-yong,HE Chen-xia,SUN Song & XIA Guang-min.Agrobacterium tumefaciens mediated choline dehydrogenase gene(betA) transfer to wheat and factors impacting on transformation[J].Journal of Shandong University,2004,39(3):104-110,115. |
| |
| Authors: | XUE Zhe-yong HE Chen-xia SUN Song & XIA Guang-min |
| |
| Abstract: | Immature embryos and embryogenic calli of wheat( Triticum aestivian L.) cultivars Jinanl77, 99P and Hesheng 3 were infected by three types of Agrobacterium LBA4404/pCDH, Agll/pUNN2 and AglO without plasmid. Transgenic plants regenerated only from embryogenic calli. PCR and PCR-Southern hybridization confirmed that transgenic plants contained foreign genes. Chromosomes of embryogenic calli infected were analysis. The elimination and breakage were more severe in the calli infected by LBA4404 than by AglO or control without infected.The factors impacted on transformation efficiency were also researched, including Agrobacterium types, wheat genotypes,explant types and period of selection etc. |
| |
| Keywords: | Wheat transformation by Agrobacterium tumefacien chromosome elimination and fragmentation transgenic plants transformation efficiency |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
