利用RAPD标记研究桂林桂花品种间的亲缘关系(英文)

<正>采用随机扩增多态性DNA(RAPD)技术,从100个随机引物中筛选出扩增效果较好的20个引物,分析桂林市23个桂花品种的基因组多态性。结果表明,20个随机引物共检测到193个位点,其中多态位点为114个,占59.1％。根据扩增结果对这些品种进行聚类分析,并构建出了树状聚类图,可将这些品种划分为4个品种群,与传统分类学结果一致。此项研究表明,以基因型为基础分析桂花品种间的区别是可行的。因此,RAPD技术为解决桂林市的桂花品种分类问题提供了重要依据。

Identifying Osmanthus fragrans Cultivars in Guilin City and Evaluating Their Genetic Relationships by RAPD Markers

Random Amplified Polymorphic DNA(RAPD) markers were applied to analyze 23 Osmanthus fragrans cultivars in Guilin City. Among the total 193 fragments generated by 20 selected primers (among 100 primers) , 114 appeared to be polymorphic(59. 1%). Cluster analysis based on the RAPD results was performed and dendrogram was constructed. These cultivars tested by RAPD were divided into 4 cultivar groups. The result was consistent with that from traditional taxonomy analysis. The RAPD study illustrated that it is possible to analyze intra-cultivar variation of O. fragrans cultivars on the basis of genotype rather than phenotype and therefore, offered significant evidences in solving taxonomic problem of O. fragrans cultivars in Guilin City.