| pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes |
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| 作者姓名： | 李冬海 阮翔 许强 王可玢 公衍道 匡廷云 张秀芳 赵南明 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China，State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China，State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China，Photosynthesis Research Center，Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China，State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China，Photosynthesis Research Center，Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China，State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China，State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China |
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| 基金项目： | Supported by the State Key Basic Research and Developm ent Plan (No. G19980 10 10 0 ) and the National Natural Science Foundation of China (No.
39890 390 ) |
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| 摘 要： | IntroductionPhotosystem II ( PSII) is a pigment- proteincomplex in the thylakoid membrane. Its reactioncenter( PSII- RC) ,which is composed of D1 andD2 proteins,generates the highly positive oxidantrequired for the oxidation of water by light- drivencharge separation. Water oxidation occurs at theMn4cluster positioned atthe center of the oxygen-evolving complex on the lumenal surface of PSII.In green plants,the highly reactive Mn4cluster isshielded by a number of extrinsic proteins( 33…
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| 关 键 词： | 植物生理学 光合体系II 叶绿素 蛋白质 pH值 荧光光谱学 |
pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes |
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| Abstract: | The effect of varying pH on the photosystem II (PSII) membrane was studied using absorption and steady-state fluorescence spectroscopy, and using a variable fluorescence technique.pH variations induced significant changes in the chlorophyll states of the PSII membrane, but no effect was seen on the chlorophyll fluorescence parameter F′v/F′m.For acidic pH conditions, protein structures of the PSII membrane were slightly altered, whilst at alkaline pH levels, large changes in the protein structure of the PSII membrane were detected.The results indicate that the microenvironment around Cys in the PSII membrane is very susceptible to alkaline pH conditions, and that in the acid (4≤pH<7) and alkaline (9≥pH>7) regions, pH variation has no effect on the protein structures of the PSII reaction center (RC). |
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| Keywords: | photosystem II (PSII) chlorophyll fluorescence pH treatment |
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