Inhibition of IgM antibody-mediated aggregation ofTrypanosoma gambiense in the presence of complement |
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| Authors: | T Takayanagi H Kawaguchi Y Yabu M Itoh K Yano |
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| Institution: | (1) Department of Medical Zoology, Nagoya City University, Medical School, Mizuho-ku, 467 Nagoya, (Japan);(2) The Institute for Microbial Diseases, Osaka University, Yamadaoka, 565 Suita City, Osaka, (Japan) |
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| Abstract: | This paper deals with the immune reaction betweenTrypanosoma gambiense and monoclonal IgM mouse antibody at equivalence with or without rabbit complement. Antibody-mediated trypanosome clumps formed in the absence of complement, and were readily dissociated by complement to become free. In the presence of complement, on the other hand,T. gambiense were not aggregated by the antibody. Free parasites adhered readily to cultured peritoneal macrophages. Complement-mediated dissociation of the clumped trypanosomes in the equivalence area released a large number of previously bound surface antigens. These antigens were capable of binding again to fresh IgM antibody. Experimental results further indicated that the complement system caused a functional alteration, changing the multivalent nature of the IgM antibody in the immune complex into a univalent one. This phenomenon is of great advantage to the infected host in clearing pathogens in vivo, as it allows more antibodies to attach to trypanosomes and subsequently initiate complement activity. |
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| Keywords: | Trypanosoma gambiense monoclonal IgM antibody immune reaction dissociation immunophagocytosis |
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