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| 毛白杨4CL基因启动子的克隆及初步功能分析 | |
| 引用本文： | 邢智峰,张安世,徐存栓,魏建华.毛白杨4CL基因启动子的克隆及初步功能分析[J].河南师范大学学报(自然科学版),2007,35(2):142-145. |
| 作者姓名： | 邢智峰 张安世 徐存栓 魏建华 |
| 作者单位： | 1. 焦作师范高等专科学校,河南,焦作,454000 2. 河南师范大学,生命科学学院,河南,新乡,453007 3. 北京农业生物技术研究中心,北京,100089 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 4CL(4-coumarate:CoA ligase,4-香豆酸:辅酶A连接酶)在植物木质素合成途径中催化羟基香豆酸生成羟基肉桂酰CoA,主要在木质部中表达,对植物木质素生物合成具重要调控作用.为研究4CL基因启动子在转基因植物中的表达特性,探索其在植物基因工程研究中的潜在应用价值,利用PCR方法从毛白杨基因组DNA中扩增得到了4CL启动子片段.序列分析表明与美洲山杨(P.tremuloids)的4CL启动子同源性为95%.采用生物信息学方法对该序列进行分析.与GUS基因融合构建双元表达载体,转化烟草的瞬时表达检测可见明显GUS活性. |
| 关 键 词： | 毛白杨 4CL基因启动子 GUS基因 |
| 文章编号： | 1000-2367(2007)02-0142-04 |
| 修稿时间： | 2007-01-11 |
Cloning of 4CL Gene Promoter from Chinese White Poplar(Populus tomentosa) and Primary Functional Characterization |
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| XING Zhi-feng,ZHANG An-shi,XU Cun-shuan,WEI Jian-hua.Cloning of 4CL Gene Promoter from Chinese White Poplar(Populus tomentosa) and Primary Functional Characterization[J].Journal of Henan Normal University(Natural Science),2007,35(2):142-145. | |
| Authors: | XING Zhi-feng ZHANG An-shi XU Cun-shuan WEI Jian-hua |
| Abstract: | 4-coumarate:CoA ligase plays an important role in the lignin metabolism in plant.In order to study the expression pattern of 4CL gene promoter and explore its potential application in plant genetic engineering,4CL promoter fragment was amplified by PCR method from Populus tomentosa genome DNA.The sequence analyses showed that it shared 95% homology with 4CL promoter in P.tremuloid at nucleotide level.This sequence was also analyzed with related Bio-Informatics software in Internet.Binary expressing vector was constructed by inserting the promoter sequence to upstream of GUS gene in pBI121,resulting in the replacement of original CaMV 35S promoter by the 4CL promoter.GUS transient expression analysis indicated that 4CL promoter could confer an expression of GUS gene. |
| Keywords: | Chinese White Poplar 4CL gene promoter GUS gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |