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| 电化学方法测定羟自由基反应速度常数 | |
| 引用本文： | 袁倬斌,邹洪. 电化学方法测定羟自由基反应速度常数[J]. 中国科学技术大学学报, 2002, 32(3): 288-292 |
| 作者姓名： | 袁倬斌 邹洪 |
| 作者单位： | 1. 中国科学技术大学研究生院化学系,北京,100039 2. 首都师范大学化学系,北京,100037 |
| 基金项目： | 国家自然科学基金 (2 9875 0 2 7，2 96 75 0 2 2 )，中科院长春应用化学研究所电分析化学开放实验室基金(OC980 0 0 3，OC2 0 0 0 0 1)，北京市教委基金，北京市自然基金 (2 2 972 0 0 3)资助项目 |
| 摘 要： | 在维生素C存在下，铁－EDTA与H2O2反应生成的羟自由基和脱氧核糖作用，在酸性条件下生成丙二醛（MDA）；MDA与甲醛、氨通过Hantzsch反应，生成产物3，5－二甲酰－1,4-氢吡啶，加入羟自由基“清除剂”，用电化学方法监测3，5－二甲酰－1，4－二氢吡啶的形成，以此求得羟自由基“清除剂”与羟自由基反应的反应速度常数。 |
| 关 键 词： | Hantzsch反应 羟自由基 反应速度常数 电化学法 3 5－二甲酰－1 4－二氢吡啶 抗氧化剂 |
| 文章编号： | 0253-2778(2002)03-0288-05 |
A Simple Assay for Determination of Rate Constants for Reactions of Hydroxyl Radicals by Electrochemical Method |
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| YUAN Zhuo-bin ,ZOU Hong. A Simple Assay for Determination of Rate Constants for Reactions of Hydroxyl Radicals by Electrochemical Method[J]. Journal of University of Science and Technology of China, 2002, 32(3): 288-292 | |
| Authors: | YUAN Zhuo-bin ZOU Hong |
| Affiliation: | YUAN Zhuo-bin 1,ZOU Hong 2 |
| Abstract: | Hydroxyl radicals, generated by reaction of an iron-EDTA complex with H 2O 2 in the presence of ascorbic acid, attack deoxyribose to form malonaldehyde (MDA) at low pH. MDA can react with formaldehyde and ammonia under weak acidic conditions to form 1,4-dihydropyrindine-3,5-dicarbaldehyde. The added hydroxyl radical "scavengers" compete with deoxyribose for the hydroxyl radicals produced and can diminish 1,4-dihydropyrindine-3,5-dicarbaldehyde formation. A rate constant for the reaction of the scavenger with hydroxyl radicals can be deduced from the inhibition of 1,4-dihydropyrindine-3,5-dicarbaldehyde formation. |
| Keywords: | Hantzsch reaction reaction rate constant hydroxyl radical electrochemical method |
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