| 羧甲基壳聚糖抗胃溃疡及其机制的实验研究 |
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| 引用本文： | 曹秀明,王佳,谢佳宁.羧甲基壳聚糖抗胃溃疡及其机制的实验研究[J].高师理科学刊,2009,29(6):64-67. |
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| 作者姓名： | 曹秀明 王佳 谢佳宁 |
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| 作者单位： | 哈尔滨商业大学,生命科学与环境科学研究中心药物所,国家教育部抗肿瘤天然药物工程研究中心,黑龙江,哈尔滨150076 |
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| 摘 要： | 为了探讨羧甲基壳聚糖的抗胃溃疡作用及其机制,将小鼠分为羧甲基壳聚糖高、中、低单个剂量组(800,400,200 mg/kg.d-1),雷尼替丁组(42 mg/kg.d-1),对照组和空白组等6个实验组,分别用消炎痛(18 mg/kg)灌胃的方法造小鼠胃溃疡模型,连续给药13 d后,计算溃疡指数和溃疡抑制率,HE染色观察组织结构,并测定超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH)的质量分数.结果表明,通过溃疡指数的计算和组织学观察,与对照组相比,羧甲基壳聚糖高、中、低剂量3组的溃疡指数明显降低(p<0.05),抑制率可分别达到50.9%,82.5%,34.39%,雷尼替丁组效果略好于羧甲基壳聚糖给药组,抑制率可达83.75%.同样,与对照组相比,连续给药羧甲基壳聚糖13 d能明显降低MDA的质量分数(p<0.05),提高SOD的活性(p<0.05),并能提高GSH的质量分数(p<0.05),其中羧甲基壳聚糖高剂量组SOD活性和GSH的质量分数均略高于雷尼替丁组.羧甲基壳聚糖可以促进消炎痛引起的小鼠实验性溃疡愈合,能够有效地清除自由基,因此其促进溃疡愈合的机制可能与其抗氧化作用有一定的关系.
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| 关 键 词： | 羧甲基壳聚糖 胃溃疡 抗氧化 |
The research of carboxymethyl chitosan on gastric ulceration in mice |
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| CAO Xiu-ming,WANG Jia,XIE Jia-ning.The research of carboxymethyl chitosan on gastric ulceration in mice[J].Journal of Science of Teachers＇College and University,2009,29(6):64-67. |
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| Authors: | CAO Xiu-ming WANG Jia XIE Jia-ning |
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| Institution: | CAO Xiu-ming,WANG Jia,XIE Jia-ning(Institute of Meteria Medica,Research Center on Life Sciences and Environmetal Sciences,Harbin University of Commerce,Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China) |
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| Abstract: | To evaluation the effects and discuss its mechanism of carboxymethyl chitosan in indomethacin-induced gastric ulceration in mice.Mice ulcerate with indomethacin(18 mg/kg)were treated up to 13 d with different doses of CM-chitosan(800,400,200 mg/kg.d-1)and ranitidine(42 mg/kg.d-1).After 13 d,the stomach tissues were excised for histological and biochemical analysis.Treatment with CM-chitosan(800 and 400 mg/kg.d-1)and ranitidine for 13 d could effectively heal the stomach ulceration as revealed from the ulcer... |
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| Keywords: | carboxymethyl-chitosan stomach ulcer antioxidant |
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