| 雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究 |
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| 引用本文： | 尚丽丽,冯碧云,叶海辉,黄辉洋,李少菁.雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究[J].厦门大学学报(自然科学版),2011,50(4):794-798. |
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| 作者姓名： | 尚丽丽 冯碧云 叶海辉 黄辉洋 李少菁 |
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| 作者单位： | 厦门大学海洋与环境学院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金项目(40776084,41076081) |
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| 摘 要： | 采用MaxVisionTM免疫组织化学方法,以兔抗人GnRH受体的多克隆抗体,对雄性三疣梭子蟹(Portunus trituberculatus)的神经器官(视神经节、脑神经节和胸神经团)进行免疫组织化学定位.结果显示:GnRH受体的免疫阳性物质在雄性三疣梭子蟹视神经节、脑神经节和胸神经团的多个部位都有较为广泛的存在,定位在神经细胞的细胞质和神经髓质中,不同神经器官GnRH受体的免疫阳性强度有一定的差异.三疣梭子蟹神经器官存在GnRH受体的免疫阳性物质,为GnRH参与其神经调节作用提供了形态学依据.
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| 关 键 词： | GnRH受体 三疣梭子蟹 神经器官 免疫组织化学 |
Immunohistochemical Study of GnRH Receptor in the Nervous Organs of the Male Swimming Crab,Portunus trituberculatus |
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| SHANG Li-li,FENG Bi-yun,YE Hai-hui,HUANG Hui-yang,LI Shao-jing.Immunohistochemical Study of GnRH Receptor in the Nervous Organs of the Male Swimming Crab,Portunus trituberculatus[J].Journal of Xiamen University(Natural Science),2011,50(4):794-798. |
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| Authors: | SHANG Li-li FENG Bi-yun YE Hai-hui HUANG Hui-yang LI Shao-jing |
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| Institution: | SHANG Li-li,FENG Bi-yun,YE Hai-hui,HUANG Hui-yang,LI Shao-jing(College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China) |
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| Abstract: | The immunohistochemical technique of MaxVisionTM method and human GnRH receptor antibody against rabbit were used in this study to find out the locations of immunoreactive substances in the nervous organs of the male swimming crab,Portunus trituberculatus.The results revealed that:the immunoreactive substances of GnRH receptor were widely detected in many parts of the optic ganglion,cerebral ganglion and thoracic ganglion mass of the male crab.The immunoreactive substances located in the cytoplasm of neuros... |
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| Keywords: | gonadotropin-releasing hormone receptor Portunus trituberculatus nervous organs immunohistochemistry |
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