小鼠NPC细胞RFX1ChIP-Seq数据分析

利用NCBI数据库中小鼠Embryonic Stem Cells(ESC)与Neural ProgenitorCells(NPC)的基因芯片结果及NPC时期的RFX1ChIP-Seq数据,进行有关RFX1的分析,结果表明:RFX1结合位点富集在1,2,4,5,7,9,11染色体上,Y染色体上最少,其他染色体上比较均衡;在基因组中结合位点分布区域主要在基因的promoter区域,约有53.2%,其次是intergentic,占22.5%,body区域,占13.1%,enhancer区域,占11.2%.说明RFX1是以结合在基因的promoter区为主要形式对目的基因进行调控.同时在DAVID数据库中用生物信息学方法探索了RFX1靶基因的生物学功能分类.

Analysis of the RFX1 ChIP-Seq Data in Mouse Embryonic Stem Cells

The datas of Neural Progenitor Cells chip and Embryonic Stem Cells ChIP-Seq from NCBI were analyzed to explore the function of RFX1. A comprehensive analysis showed that RFX1 binding sites spread across each chromosome. Most of them resided in chromosomes 1, 2, 4, 5, 7, 9 and 11, but the least in chromosome Y. And genome wide localization prediction showed that RFX1 binding sites ubiquitously and generally existed throughout the genome. 53.2% of these sequences were distributed in promoter, 22.5 % in the intergentic, 13.1% in the body, and 11.2% in the enhancer in the mouse genome. In addition, the biological processes of RFX1 target genes were analyzed. The majority of the affected biological processes were mainly cellular and metabolic.