昆明西山野生蘡薁葡萄资源及其遗传多样性分析

调查了昆明西山野生蘡薁葡萄(Vitis bryoniaegolia Bge)的分布特征,并利用RAPD分子标记对其5个群体的遗传多样性进行了分析.采用5个RAPD引物扩增,共产生71条DNA条带,其中多态条带68条.在物种水平上,蘡薁葡萄多态位点百分率(PPB)为95.77%,Nei's基因多样性指数(H)为0.3442,Shannon's多样性信息指数(Hsp)为0.5100;在群体水平上,PPB差异较大(35.21%～84.51%),平均值为60.85%,H平均为0.2234,Ho平均为0.3305.各群体间的Nei's遗传一致度(I)范围为0.7171～0.9489.基于Nei's遗传多样性分析得出的群体间遗传分化系数Gst=0.3269,表明西山蘡薁葡萄的遗传多样性主要来源与群体内而不是群体间.Mantel检测发现,群体间的遗传距离和物理距离之间呈显著的正相关(r=0.981,P=0.0190.05).

A study on the distribution and genetic diversities of wild graperesources Vitis bryoniaegolia in Xishan mountain,Kunming

The ecological distribution characters of Vitis bryoniaeglia Beg from Xishan mountain in Kunming were investigated,the genetic diversities based on the RAPD markers were studied in 5 groups of Vitis bryoniaegolia Bge.The results indicated that 68 diversified loci in total 71 DNA bands were amplified by using 5 random primers.At species level,the percentage of polymorphic bands (PPB), Nei's gene diversity (H) and the Shannon's information index ( H_sp ) were 95.77%,0.3442 and 0.5100,respectively.While at groups level ,the average PPB was 60.85 % (varied from 35.21 %—84.51%).The average H and H_o were 0.2234 and 0.3305,respectively.The coefficient of genes differentiation among groups ( G_st ) based on Nei's genetic distance was 0.3269,which implied that the gene variation was originated mainly within the groups (77.31%) other than among (32.69%) the groups.Genetic identity (I) of the 5 groups was varied from 0.7171 to 0.9489.The geographical distance was significant correlated with the genetic distance based by the Mantel analysis as r=0.981,P=0.019<0.05.