三七多糖的微波辅助提取工艺

采用正交试验法优化了三七多糖的微波提取工艺,采用激光粒度分析法探讨了提取机理,分析了多糖的单糖组成,并测定了多糖的溶解性。三七多糖的较佳微波辅助提取工艺条件为微波处理时间8min、浸泡时间60min、液固比30mL/g、粒度200目。微波辅助提取的多糖提取率(21.0mg/g)显著高于传统提取(12.7mg/g)。三七多糖的Molish反应呈阳性,多糖酸水解产物的薄层色谱呈现出3个斑点,分别与葡萄糖、半乳糖和阿拉伯糖相对应。微波辅助提取对三七多糖的提取效果明显优于传统提取,其机理可能是微波处理促进了三七样品的水化和溶胀过程,使样品结构变得松散、膨胀,从而促进了多糖从样品中扩散和溶出。三七多糖主要由葡萄糖、半乳糖和阿拉伯糖3种单糖构成,pH值对其溶解性影响较大。

Microwave Assisted Extraction of Polysaccharides from Panax notoginseng

Microwave assisted extraction of polysaccharides from Panax notoginseng was optimized using single factor experiments and the orthogonal test. The mechanism underlying microwave assisted extraction was identified by laser diffraction particle sizing analysis, and the monosaccharide composition of polysaccharides was characterized by thin-layer chromatography. The optimal conditions for microwave assisted extraction were obtained:microwave duration of 8min, soaking time of 60min, ratio of liquid to solid of 30mL/g, and particle size of 200 meshes.The extraction yield of microwave assisted extraction (21.0mg/g) was significantly higher than that of conventional extraction (12.7mg/g). Thin-layer chromatography and solubility of polysaccharides were studied. Microwave assisted extraction was superior to conventional extraction in terms of the extraction yield. The possible reason was that microwave radiation facilitated the swelling of plant samples, and their structure became loose, which led to the exudation of polysaccharides. Polysaccharides from P. notoginseng were mainly composed of three monosaccharides, which were glucose, galactose and arabinose, while the pH value greatly affected solubility of polysaccharides from P. notoginseng.