| 菜豆(双青35号)再生体系的建立 |
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| 引用本文： | 包英华,白音,王羽梅,粟艳梅,罗靖冬,余红媛. 菜豆(双青35号)再生体系的建立[J]. 韶关学院学报, 2005, 26(12): 74-77 |
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| 作者姓名： | 包英华 白音 王羽梅 粟艳梅 罗靖冬 余红媛 |
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| 作者单位： | 韶关学院,英东生物工程学院,广东,韶关,512005 |
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| 基金项目： | 广东省自然科学基金资助项目（021272）. |
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| 摘 要： | 报道了双青35号菜豆不同外植体在含有不同激素的培养基上诱导、分化及生根情况,并建立其高效再生体系．研究结果表明：愈伤组织诱导和增殖培养基为MS＋6-BA2．0mg/L＋NAA2．0mg/L,不定芽再生培养基为MS＋6-BA2．0mg/L＋NAA0．2mg/L,生根培养基为1／2MS＋IAB3．0mg/L＋0．1％活性炭是较佳的．
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| 关 键 词： | 双青35号菜豆 不同外植体 再生体系 |
| 文章编号： | 1007-5348(2005)12-0074-04 |
| 收稿时间： | 2005-06-01 |
| 修稿时间： | 2005-06-01 |
Establishment for a Regeneration System from Phaseolus vulgaris cv. "Shuangqing 35" |
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| BAO Ying-hua,BAI Yin,WANG Yu-mei,SU Yan-mei,LUO Jing-dong,YU Hong-yuan. Establishment for a Regeneration System from Phaseolus vulgaris cv. "Shuangqing 35"[J]. Journal of Shaoguan University(Social Science Edition), 2005, 26(12): 74-77 |
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| Authors: | BAO Ying-hua BAI Yin WANG Yu-mei SU Yan-mei LUO Jing-dong YU Hong-yuan |
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| Affiliation: | Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, Guangdong, China |
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| Abstract: | Differentiation and rooting of different explants of Phaseolus vulgaris cv."Shuangqing 35" were described on different medium in which contained different hormones and a high efficient regeneration system was established.The result showed that the most suitable medium of inducing and proliferating callus is MS+6-BA 2.0 mg/L +NAA 2.0 mg/L,medium of regenerating adventitious bud is MS+6-BA 2.0 mg/L+NAA0.2 mg/L,rooting medium is 1/2MS+IAB3.0 mg/L+0.1% mg/L active carbon. |
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| Keywords: | Phaseolus vulgaris cv." Shuangqing 35" ,different explants,regeneration system |
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