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Cu2+对两种海洋微藻生长和叶绿素荧光特性的影响
引用本文:姜恒,吴斌,阎冰,吴志强,邢永泽.Cu2+对两种海洋微藻生长和叶绿素荧光特性的影响[J].广西科学,2013,20(1):60-65.
作者姓名:姜恒  吴斌  阎冰  吴志强  邢永泽
作者单位:1. 广西红树林保护重点实验室,广西红树林研究中心,广西北海536000;桂林理工大学,广西桂林541004
2. 广西红树林保护重点实验室,广西红树林研究中心,广西北海536000
3. 桂林理工大学,广西桂林,541004
基金项目:广西自然科学基金北部湾重大专项(2011GXNSFE018001);国家海洋公益性行业科研专项(201005012);广西科学研究与技术开发计划项目(桂科合1010019-23);广西科学院科研业务费(11YJ24HS03)资助
摘    要:运用水样叶绿素荧光仪(WATER-PAM)研究Cu2+胁迫下,湛江叉鞭金藻(Dicrateria zhanjiangensis)和球等鞭金藻3011(Isochrysis galbana MACC/H59)叶绿素荧光特性的变化,同时监测微藻的生长情况.结果就叶绿素荧光参数而言,湛江叉鞭金藻和球等鞭金藻3011的叶绿素荧光参数Fv/Fm,Fv/F0,Yield和ETR均随着Cu2+浓度的增大而明显降低;就生长情况而言,随着Cu2+浓度的增大,两种海洋微藻细胞密度的增长明显变缓.在24 h时Cu2+对湛江叉鞭金藻的毒性大于球等鞭金藻3011,48~96 h时Cu2+对球等鞭金藻3011的毒性大于湛江叉鞭金藻,Cu2+的毒性随着胁迫时间的延长而逐步增大.

关 键 词:湛江叉鞭藻  球等鞭金藻3011  叶绿素荧光  细胞密度  Cu2+
收稿时间:2012/10/23 0:00:00
修稿时间:2012/11/23 0:00:00

Effects of Cu2+ on the Growth and Chlorophyll Fluorescence of Two Species of Marine Microalgae
JIANG Heng,WU Bin,YAN Bing,WU Zhi-qiang and XING Yong-ze.Effects of Cu2+ on the Growth and Chlorophyll Fluorescence of Two Species of Marine Microalgae[J].Guangxi Sciences,2013,20(1):60-65.
Authors:JIANG Heng  WU Bin  YAN Bing  WU Zhi-qiang and XING Yong-ze
Institution:1 (1.Guangxi Key Lab for Mangrove Conservation,Guangxi Mangrove Research Center,Beihai,Guangxi,536000,China;2.Guilin University of Science and Technology,Guilin,Guangxi,541004,China)
Abstract:The changes of the fluorescence parameters of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 exposed to Cu2+ were investigated by means of the chlorophyll fluorescence technology(Water-PAM), and the growth of these two species of marine microalgae was observed. The results showed that all fluorescence parameters (Fv/Fm, Fv/F0, Yield and ETR) of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress. The growth of cell density for Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress, and it can be expressed as Dicrateria zhanjiangensis > Isochrysis galbana MACC/H59 in the 24h while it can be expressed as Isochrysis galbana MACC/H59 > Dicrateria zhanjiangensis during 48h to 96h. The toxicity of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 increased with the increase of exposure time.
Keywords:Dicrateria zhanjiangensis  Isochrysis galbana  chlorophyll fluorescence  cell density  Cu2+
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