| 青岛文昌鱼发育信号传导相关基因hedgehog的克隆 |
| |
| 引用本文： | 毛炳宇,张燕君,张红卫.青岛文昌鱼发育信号传导相关基因hedgehog的克隆[J].山东大学学报(理学版),2001,36(1):95-100. |
| |
| 作者姓名： | 毛炳宇 张燕君 张红卫 |
| |
| 作者单位： | 山东大学生命科学院, |
| |
| 基金项目： | 国家自然科学基金(39670357)、高等学校博士学科点专项科研基金、山东省自然科学基金(Y96D08061) |
| |
| 摘 要： | hedgehog家族基因在动物胚胎多种发育过程的信号传导中起着关键作用.采用简并引物、套式PCR和RT-PCR方法获得青岛文昌鱼hedgehog基因片段，并对其所推测的氨基酸序列与hh基因家族成员小鼠Shh、Ihh、Dhh，鸡、爪蟾和斑马鱼Shh及果蝇hh等的相应片段进行同源性分析.它们的同源性分别为56％，53％，50％，53％，53％，53％和45％.研究结果支持文昌鱼具有1个，可能也仅有1个hedgehog家族基因.
|
| 关 键 词： | 文昌鱼 hedgehog 克隆 同源性分析 |
| 文章编号： | 0559-7234(2001)01-0095-06 |
| 修稿时间： | 2000年1月12日 |
CLONING OF QINGDAO
AMPHIOXUS HEDGEHOG GENE,A MOLECULE INVOLVED IN INTERCELLULAR SIGNALING OF DEVELOPMENT |
| |
| MAO Bing-yu,ZHANG Yan-jun,ZHANG Hong-wei.CLONING OF QINGDAO
AMPHIOXUS HEDGEHOG GENE,A MOLECULE INVOLVED IN INTERCELLULAR SIGNALING OF DEVELOPMENT[J].Journal of Shandong University,2001,36(1):95-100. |
| |
| Authors: | MAO Bing-yu ZHANG Yan-jun ZHANG Hong-wei |
| |
| Abstract: | The degenerate primers were designed according to the conserved sequences of hedgehog gene family.A single hh gene fragment of Qindao amphioxus was cloned by PCR and RT-PCR.The homology analysis of deduced amino acid sequence showed that Qingdao amphioxus hh had 56%,53%,50%,53%,53%,53% and 45% similarities with mouse Shh,Ihh,Dhh, chick,Xenopus,zebrafish Shh,and Drosophila hh respectively.The results showed that amphioxus contained one and probably only one hh gene. |
| |
| Keywords: | amphioxus hedgehog cloning homology analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
