| 水稻热激蛋白基因（HSP70）的PCR法快速分析 |
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| 引用本文： | 宗晖,刘晓斐.水稻热激蛋白基因（HSP70）的PCR法快速分析[J].复旦学报(自然科学版),1996,35(6):649-655. |
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| 作者姓名： | 宗晖 刘晓斐 |
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| 作者单位： | 复旦大学生物化学系 |
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| 基金项目： | 国家水稻基因组研究计划 |
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| 摘 要： | 通过比较几个已知ＨＳＰ７０的ｃＤＮＡ顺序，用ＰＣＧＥＮＥ软件中的ＰＣＲＰＬＡＮ程序设计出一对简并引物，然后从水稻（广陆矮４号）总ＤＮＡ中扩增出ＨＳＰ７０的基因片段并将其克隆到ｐＢＬＵＥＳＣＲＩＰＴ载体中。在完成了对克隆的序列测定之后，用ＰＣＧＥＮＥ中有关程序对其进行同源分析，并对其他已知的ＨＳＰ７０作了一些分类及分子进化方面的探讨。
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| 关 键 词： | 水稻 热激蛋白 热激蛋白基因 聚合酶链反应 |
Rapid analysis of rice HSP70 gene by PCR |
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| Zong Hui,Liu Xiaofei,Jiang Ying,Cao Kaiming.Rapid analysis of rice HSP70 gene by PCR[J].Journal of Fudan University(Natural Science),1996,35(6):649-655. |
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| Authors: | Zong Hui Liu Xiaofei Jiang Ying Cao Kaiming |
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| Institution: | Department of Biochemistry |
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| Abstract: | Based on several available HSP70 cDNA sequences, a pair of degenerated primers was designated with the help of PCRPLAN program in PCGENE package. Then several HSP70 gene fragments of rice were cloned by polymerase chain reaction (PCR) from total DNA of rice and sequenced. These fragments were subjected to homology analysis,together with other known HSP70 sequences from PCGENE package,and their implications to the grouping and molecular evolution of HSP70 gene family are discussed. |
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| Keywords: | rice heat shock protein(HSP70)PCR degenerated primer |
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