| 人巨细胞病毒临床低传代病毒株UL97 基因的克隆与分析 |
| |
| 引用本文： | 王波,李月琴,田传军,张纯青,苏运贞,何华坤,周天鸿.人巨细胞病毒临床低传代病毒株UL97 基因的克隆与分析[J].暨南大学学报,2005,26(5):694-699. |
| |
| 作者姓名： | 王波 李月琴 田传军 张纯青 苏运贞 何华坤 周天鸿 |
| |
| 作者单位： | 暨南大学生物工程学系,广东,广州,510632;广州医学院第一附属医院,广东,广州,510120;暨南大学生物工程学系,广东,广州,510632;广州医学院第一附属医院,广东,广州,510120 |
| |
| 基金项目： | 国家自然科学基金资助项目（30370776）;广东省医学科学基金资助项目（A2004272）;广州市属高校科技计划资助项目（2004-1045）;广州医学院科研基金资助项目（03-K-15）. |
| |
| 摘 要： | 分离人巨细胞病毒（HCMV）临床低传代GZ02病毒株，并根据GenBank提供的实验室标准病毒株HCMV AD169磷酸转移酶基因UL97 DNA序列及有关文献设计引物，从HCMV GZ02病毒株基因组DNA中通过PCR扩增UL97基因，并克隆至pGEM3Z质粒载体．重组质粒经测序鉴定，发现HCM VGZ02病毒株UL97基因序列保守区域与HCMV AD169 UL97长度完全一致，但发生G205A、G761A、T823C、T1173C、T1282C、T1334C、T2106C等7个位点的碱基突变，涉及到编码氨基酸E69K，S275P，C428R和F445S位点发生改变。
|
| 关 键 词： | 人巨细胞病毒 野生型 UL97基因 突变 克隆 序列分析 |
| 文章编号： | 1000-9965(2005)05-0694-06 |
| 收稿时间： | 09 13 2004 12:00AM |
| 修稿时间： | 2004年9月13日 |
Cloning and analysis of HCMV clinical low passage strain UL97 gene |
| |
| WANG Bo,LI Yue-qin,TIAN Chuan-jun,ZHANG Chun-qing,SU Yun-zhen,HE Hua-kun,ZHOU Tian-hong.Cloning and analysis of HCMV clinical low passage strain UL97 gene[J].Journal of Jinan University(Natural Science & Medicine Edition),2005,26(5):694-699. |
| |
| Authors: | WANG Bo LI Yue-qin TIAN Chuan-jun ZHANG Chun-qing SU Yun-zhen HE Hua-kun ZHOU Tian-hong |
| |
| Institution: | 1. Department of Biotechnology, Jinan University, Guangzhou 510632, China; 2. Department of Pediatrics,the First Teaching Hospital of Guangzhou Medica,Guangzhou 510120, China |
| |
| Abstract: | Separating the HCMV clinical low passage GZ02 strain from the patients who infect HCMV after Allo HSCT, we amplified the UL97 DNA fragment by PCR with the primers according to laboratory standard strain AD169 UL97 complete genomic provided in CenBank and related literatures. The UL97 DNA fragment was inserted into pGEM3Z vector and the re combinant plasmids were subsequently sequenced and analyzed. The conserved regions of U197 genomic sequence were correspond with AD169 UL97 reported in GenBank approximately. But there are 7 points mutations in sites of 205 (G205A), 761 (G761A), 823 (T823C), 1173 (T11730,1282(T12820,1334(T1334C),2106(T2106C) of the clinical isolate HCMV GZ02, which result in amino acid alterations, such as codons at 69(E69K) ,275(S275P) ,248 (C428R) and 445(F445S). |
| |
| Keywords: | human cytomegalovirus clinical low passage UL97 mutation clone sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
