| HMG＿（14）单克隆抗体杂交瘤株的建立 |
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| 引用本文： | 童苏湘,吴鹤龄.HMG＿（14）单克隆抗体杂交瘤株的建立[J].北京大学学报(自然科学版),1994(6). |
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| 作者姓名： | 童苏湘 吴鹤龄 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 以北京鸭红细胞提取的ＨＭＧｓ（ＨＭＧ＿１，ＨＭＧ＿（２ａ），ＨＭＧ＿（２ｂ），ＨＭＧ＿（１４），ＨＭＧ＿（１７））免疫Ｂａｌｂ／ｃ小鼠，取其脏压细胞与Ｓｐ２／０（小鼠骨髓瘤细胞）融合，经选择培养，阳性筛选，克隆化以及冻存复苏，获得了三株稳定分泌抗ＨＭＧ＿（１４）的杂交瘤细胞株（１Ｃ＿（１２），１Ｏ＿３和２Ｆ＿２），经ＷｅｓｔｅｒｎＢｌｏｔ鉴定，与其它几种ＨＭＧ无反应，抗体为ＩｇＧ＿１亚型。腹水ＨＭＧ＿（１４）单克隆抗体经ＳｅｐｈａｄａｘＣＬ－４Ｂ亲和柱得到了纯化。
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| 关 键 词： | 单克隆抗体，高泳动率组的非组蛋白 |
Greating the Hybridomas of Monoclonal Antibody Against HMG_(14) |
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| TONG Suxiang,WU Heling.Greating the Hybridomas of Monoclonal Antibody Against HMG_(14)[J].Acta Scientiarum Naturalium Universitatis Pekinensis,1994(6). |
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| Authors: | TONG Suxiang WU Heling |
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| Abstract: | Spleen cells from Balb/c mice immunied with duckred blood cell HMG were fused with murine myeloma cell line Sp2/0. By selective culture,screening and subcloning three hybridomas 1C_(12),1O_3and2F_2 were obtained which secreted mono-clonal antibody(MoAb)against HMG stably. 1C_(12),1O_3 and 2F_2 MoHb had strong reaction with HMG,but not react with oth-er HMG and H_(12). These three moAbs are IgG_1 pattern. |
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| Keywords: | monoclonal antibody HMG non-histon protein |
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