| EGFP-IgG4融合基因真核表达载体的构建及其表达 |
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| 引用本文： | 郑立恒,许松梅,何波,张青,熊华辉,刘全胜. EGFP-IgG4融合基因真核表达载体的构建及其表达[J]. 四川大学学报(自然科学版), 2007, 44(3): 711-714 |
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| 作者姓名： | 郑立恒 许松梅 何波 张青 熊华辉 刘全胜 |
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| 作者单位： | 四川大学生命科学学院,成都,610041;中国医学科学院输血研究所,成都,610081;四川大学生命科学学院,成都,610041 |
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| 摘 要： | 构建增强型绿色荧光蛋白（Enhanced Green Fluorescent Protein, EGFP）和IgG4基因的融合蛋白真核表达载体pMM-EGFP-IgG4/WG，转染至中华仓鼠卵巢细胞(Chinese Hamster Ovary，CHO)中成功表达，并发出绿色荧光，证明pMM-EGFP-IgG4/WG是一种良好的生产分泌型外源融合蛋白的阳性对照.
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| 关 键 词： | CHO细胞 转染 EGFP-IgG4融合基因 |
| 文章编号： | 0490-6756（2007）03-0711-04 |
| 修稿时间： | 2006-08-102007-01-10 |
Construction and expression of eukaryotic expression vector containing EGFP-IgG4 fusion gene |
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| ZHENG Li-heng,XU Song-mei,HE Bo,ZHANG Qing,XIONG Hua-hui and LIU Quan-sheng. Construction and expression of eukaryotic expression vector containing EGFP-IgG4 fusion gene[J]. Journal of Sichuan University (Natural Science Edition), 2007, 44(3): 711-714 |
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| Authors: | ZHENG Li-heng XU Song-mei HE Bo ZHANG Qing XIONG Hua-hui LIU Quan-sheng |
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| Affiliation: | 1. College of Life Science, Sichuan University, Chengdu 610041, China; 2. Institute of Bled Transfusion, CAMS, Chengdu 610081, China |
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| Abstract: | The eukaryotic expression vector pMM-EGFP-IgG4/WG was constructed for determination of expression of enhanced green fluorescent protein (EGFP) gene in Chinese Hamster Ovary (CHO) cells. It was demonstrated that EGFP gene was expressed in CHO cells, implying that EGFP was a good positive comparison of producing secreted outside fusion protein. |
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| Keywords: | CHO cell transfection EGFP-IgG4 fusion gene |
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