| 灵芝漆酶活性测定条件的优化 |
| |
| 引用本文： | 魏佳,吴天祥,杨海龙.灵芝漆酶活性测定条件的优化[J].井冈山大学学报（自然科学版）,2011(5):51-54. |
| |
| 作者姓名： | 魏佳 吴天祥 杨海龙 |
| |
| 作者单位： | 贵州大学生命科学学院;温州大学生命与环境科学学院; |
| |
| 基金项目： | 浙江省自然科学基金项目(Y3100711) |
| |
| 摘 要： | 本研究以2,2’-连氮-二(3-乙基苯并噻唑-6-磺酸)(简称ABTS)为底物,测定灵芝漆酶活性的方法,探讨了酶反应温度、缓冲液及其pH值等条件对灵芝漆酶催化反应的影响。结果表明,ABTS法测定灵芝漆酶活性的最佳条件为:以pH3的0.05mol/L的磷酸氢二钠-柠檬酸溶液为反应溶液,反应温度65℃。
|
| 关 键 词： | 漆酶 灵芝 测定 优化 |
OPTIMIZATION OF DETERMINATION CONDITION OF LACCASE FROM GANODERMA LUCIDUM |
| |
| WEI Ji,WU Tian-xiang,YANG Hai-long.OPTIMIZATION OF DETERMINATION CONDITION OF LACCASE FROM GANODERMA LUCIDUM[J].Journal of Jinggangshan University(Natural Sciences Edition),2011(5):51-54. |
| |
| Authors: | WEI Ji WU Tian-xiang YANG Hai-long |
| |
| Institution: | WEI Jia1,2,WU Tian-xiang1,YANG Hai-long2(1.School of Life Science,Guizhou University,Guiyang,Guizhou 550025,China,2.School of Life&Environmental Sciences,Wenzhou University,Wenzhou,Zhejiang 325035,China) |
| |
| Abstract: | The determination condition of Ganoderma lucidum laccase was studied using ABTS as substrate, in which the effects of reaction temperature, buffer type, buffer concentration, and pH on laccase catalytic reaction were explored. The results showed the G lucidum laccase could be determined in 0.05 mol/L Na2HPO4-citric acid buffer with pH 3 at 65℃ by ABTS method. |
| |
| Keywords: | laccase Ganoderma lucidum determination optimization |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《井冈山大学学报（自然科学版）》浏览原始摘要信息 |
| 点击此处可从《井冈山大学学报（自然科学版）》下载免费的PDF全文 |
|
