纳米TiO2材料的环境与健康效应初探

小鼠采用非暴露式气管内注人法注入颗粒悬液建立动物模型,定期染毒,在第10 d处死小鼠,并进行肺泡灌洗,测定肺泡灌洗液(BALF)中各项指标以及肺脏器系数.纳米T iO210 m g/kg bw组以上各指标均高于对照组和纳米T iO21 m g/kg bw组,差异显著(P<0.01或P<0.05).纳米T iO210 m g/kg bw组脏器系数、LDH活力、HYP含量均高于常规T iO210 m g/kg bw组,差异显著(P<0.01或P<0.05);虽然纳米T iO210 m g/kg bw组白细胞数目高于常规T iO210 m g/kg bw组(7.47×105),但差异无显著性.在本实验条件下,纳米T iO2颗粒对小鼠肺的急性损伤作用比常规T iO2更严重,对生物体健康构成潜在威胁,为研究纳米T iO2对人体及环境的潜在性影响奠定实验基础.

Research on Environment and Health Activities of Nanosized TiO2

Animal models were worked out by injecting granule solution into the lung of rats through trachea.All rats were intratracheally instilled every three days.At the tenth day,all rats were sacrificed and there lungs were lavaged.The indexes in bronchoalveolar lavage fluid were examined.At last,Lung/body coefficient was measured.All indexes of nano-10mg/kg bw group were obviously higher than the control group and the group of nano-1 mg/kg bw(P<0.01 or P<0.05).At the same dose,three indexes(lung/body coefficient,activities of LDH,contents of HYP) of nano-10 mg/kg bw group were higher than normal-10mg/kg bw group(P<0.01 or P<0.05).The amount of leukocyte were also higher than normal-10mg/kg bw group,but the difference was not statistical significance.Under this experimental condition,the effect of lung injury is more severity in nanosized TiO_2 than normal TiO_2,it is a threat against organism's health.The experiment laid a foundation of research on the potential influence of nanosized TiO_2 to human and environment.