Carboxypeptidase E and thrombospondin-1 are differently expressed in subcutaneous and visceral fat of obese subjects |
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| Authors: | Ramis J M Franssen-van Hal N L W Kramer E Llado I Bouillaud F Palou A Keijer J |
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| Institution: | (1) Department of Fundamental Biology and Health Sciences, University of the Balearic Islands, Palma de Mallorca (Spain), ES;(2) Effects of Food Bioactives Unit, RIKILT, Bornesteeg 45, PO Box 230, 6700 AE Wageningen (The Netherlands), Fax +31 317 417717, e-mail: j.keijer@rikilt.wag-ur.nl, NL;(3) Ceremod C.N.R.S., Meudon (France), FR |
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| Abstract: | The aim of this study was to identify candidate genes for visceral obesity by screening for genes strongly differentially
expressed between human subcutaneous and visceral adipose depots. A cDNA microarray with human adipose-derived cDNAs was used
as an initial screening to identify genes that are potentially differentially expressed between human subcutaneous and visceral
abdominal fat tissues. For the two best candidates, carboxypeptidase E (CPE) and thrombospondin-1 (THBS1) (EST N72406), real-time
RT-PCR was performed to confirm their depot specific expression in extremely obese individuals. Both genes appeared to be
strongly differentially expressed, having a higher expression in the visceral depot than in the subcutaneous one. For THBS1,
the difference in expression between the depots was greater in women than in men. The involvement of CPE and THBS1 in obesity
allows us to suggest that the physiological processes controlled by these genes contribute to depot and gender-related differences
in the metabolic complications of obesity.
Received 7 August 2002; received after revision 19 Septemer 2002; accepted 24 September 2002 |
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| Keywords: | , Visceral adiposity, real-time RT-PCR, CPE, THBS1, differential gene expression, |
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