沙田柚花粉管在花柱中的生长途径及其识别

应用辣根过氧化物酶酶标ConA电镀细胞化学方法观察了沙田柚不同发育时期1／2花柱通道细胞的结构与功能，结果表明，四分体至双核期，是壁物质，胞间质和糖蛋白合成期，双核至开花期，通道细胞分化成熟，壁物质降解，角质层下腔形成并贮存胞间质，糖蛋白呈颗粒状定位于质膜与壁之间电子透明层中，授粉后1－3d，通道细胞逐步衰老，糖蛋白定位地纤维外壁，未见胞间隙形成，花粉管是从几个相邻通道细胞角隅处的角质层下腔和纤维外壁之间生长通过，自交花粉管因胼胝质在壁上添加而停止生长，花粉管生长道路决定于引导组织细胞壁的厚薄及角质层破裂时间，此外，讨论了纤维外壁上的糖蛋白对花粉管与花柱的识别作用。

THE GROWTH WAY OF POLLEN TUBES IN THE STYLE OF SHATINYU AND ITS DISCERNMENT

By the cytochemical method using Concanavalin A-Horseradish peroxidase conjugates,the structure and function of the 1/2 stylar canal cells in the various growth stages of Shatinyu(Citrus grandis var.shatinyu Hort.)were observed.From tetrad to dikaryaphase was stage of synthesizing the cell wall and intercellular substances and glycoproteins.From dikaryaphase to flowering,the canal cells differentiated to mature.The cell wall substances were degraded.The caticular lower-cavity formed and stored the intercellular substances.The glycoproteins showed granular and were located in the electronic transparent-layer between the plasmolemma and cell wall.In the 1 to 3 days after pollinating,the canal cells were nearing decrepitude.The glycoproteins were located in the outer cellulose wall and the intercellular space didn't form.The pollen tubes grew to pass between the lower cavity of the angular caticle of several adjacent canal cells and outer cellulose wall.The self-pollinated pollen tubes stopped growing as the callose added on the cell wall.The experimental results showed the growth way of pollen tubes was determined by the cell wall thickness of guidance tissue and reptured-time of caticule.The discerning action of the glycoproteins on the cellulose wall for the pollen tubes and style was discussed.