| 海甘蓝硫甙合成关键酶S-GT基因克隆及种子特异性hpRNAi载体构建 |
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| 引用本文： | 李长春,乔富兴.海甘蓝硫甙合成关键酶S-GT基因克隆及种子特异性hpRNAi载体构建[J].孝感学院学报,2008,28(6). |
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| 作者姓名： | 李长春 乔富兴 |
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| 作者单位： | 1. 孝感学院,生命科学技术学院,湖北,孝感,432000
2. 湖北大学,生命科学学院,湖北,武汉,430062 |
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| 基金项目： | 湖北省教育厅国际合作重点项目
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Swedish Research Links Programme
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| 摘 要： | 根据甘蓝型油菜S-GT(thiohydroximate S-glucosyltransferase)基因cDNA序列设计引物,以海甘蓝总DNA为模板进行PCR扩增.获得S-GT基因全长.克隆的海甘蓝S-GT序列与甘蓝型油菜序列相比,除74 bp的内含子部分外有92个碱基的差别,相似性高达93.4%.分析显示该序列均有完整的开放阅读框,并表明所克隆的海甘蓝S-GT序列编码465个氨基酸,在第10个位点上比甘蓝型油菜序列少一个丙氨酸(A),总共有23个氨基酸不同,相似性为95.06%.根据获得的基因序列设计引物扩增出同一基因序列相同但是带有不同酶切位点的两个片段,将两个片段反向插入到已构建的带有种子特异表达载体内含子的两端,成功构建了海甘蓝S-GT基因的种子特异性hpRNAi载体,为特异性降低海甘蓝的种子硫甙奠定了基础.
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| 关 键 词： | 海甘蓝 硫甙 hpRNAi载体 |
Cloning and hpRNAi Vector Construction for Genes of Thiohydroximate S-glucosyltransferase in Crambe abyssinica |
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| Li Changchun,Qiao Fuxing.Cloning and hpRNAi Vector Construction for Genes of Thiohydroximate S-glucosyltransferase in Crambe abyssinica[J].JOURNAL OF XIAOGAN UNIVERSITY,2008,28(6). |
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| Authors: | Li Changchun Qiao Fuxing |
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| Institution: | Li Changchun1,Qiao Fuxing2(1.College of Life Science , Technology,Xiaogan University,Xiaogan,Hubei 432000,China,2.School of Life Science,Hubei University,Wuhan,Hubei 430062,China) |
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| Abstract: | Thiohydroximate S-glucosyltransferase(S-GT) plays a key role in the process of glucosinolate(GS) biosynthesis.In this research,PCR primers were designed according to the cDNA sequence of S-GT gene in Brassica napus and full length of the S-GT genes in Crambe abyssinica were obtained by using the genomic DNA as PCR templates.Sequence alignment revealed that CaSGT gene we cloned had an intron of 74 bp and coded a protein of 465 amino acids and shared a similarity of 93.4% at the DNA sequence level and a simil... |
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| Keywords: | thiohydroximate S-glucosyltransferase |
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