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仙客来组织培养快繁技术研究
引用本文:邹迎春,覃大吉,帅超群,曾凡忠,向极钎,杨永康.仙客来组织培养快繁技术研究[J].湖北民族学院学报(哲学社会科学版),2011,29(1).
作者姓名:邹迎春  覃大吉  帅超群  曾凡忠  向极钎  杨永康
作者单位:邹迎春,帅超群,曾凡忠,ZOU Ying-chun,SHUAI Chao-qun,ZENG Fan-zhong(恩施清江生物工程有限公司,湖北,恩施,445000);覃大吉,向极钎,杨永康,QIN Da-ji,XIANG Ji-qian,YANG Yong-kang(恩施清江生物工程有限公司,湖北,恩施,445000;湖北省农业科技创新中心鄂西综合试验站,湖北,恩施,445000)
基金项目:农业高新技术示范园建设项目,恩施州农科院青年创新基金课题
摘    要:将仙客来的块茎、叶片、根尖、花梗外植体,在含有不同质量浓度植物激素的几种培养基中培养,根据培养结果,筛选出最佳诱导和增殖培养基的配方;同时,在经过消毒处理的不同成份的无土栽培基质中,进行试管苗的炼苗和壮苗培养,研究其健身栽培技术.结果表明,仙客来的离体繁植以其块茎为最佳,丛芽的增殖以MS+NAA0.1 mg/L+6-BA2.0mg/L+蔗糖30g/L为最佳,生根培养基以1/2MS+NAA0.3mg/L+蔗糖30g/L为最佳.泥炭∶蛭石∶珍珠岩(5∶3∶2)混合是仙客来试管苗最佳健身栽培基质,移栽成活率达98%.

关 键 词:仙客来  组培  快繁

Study on Tissue Culture Technique for Rapid Propagation of Cyclamen persicum
ZOU Ying-chun,QIN Da-ji,SHUAI Chao-qun,ZENG Fan-zhong,XIANG Ji-qian,YANG Yong-kang.Study on Tissue Culture Technique for Rapid Propagation of Cyclamen persicum[J].Journal of Hubei Institute for Nationalities(Natural Sciences),2011,29(1).
Authors:ZOU Ying-chun  QIN Da-ji    SHUAI Chao-qun  ZENG Fan-zhong  XIANG Ji-qian  YANG Yong-kang
Institution:ZOU Ying-chun1,QIN Da-ji1,2,SHUAI Chao-qun1,ZENG Fan-zhong1,XIANG Ji-qian1,YANG Yong-kang1,2(1.Enshi Qingjiang Bio-Engineering Co.,Ltd.,Enshi 445000,China,2.Enshi Comprehensive Experiment Station of Hubei Provincial Agriculture S&T Innovation Center,China)
Abstract:Tubers,leaves,root tips and flower stalks of Cyclamen persicum were cultured as explants in different culture mass concentration media,containing different concentration of plant hormones.According to the culture results,the most suitable explant,medium formula of induction and proliferation were selected.Meanwhile,the plantlets were transplanted in disinfected soilless culture substrate of different composition,for acclimatization and seedling culture,to study the fitness cultivation techniques.The results...
Keywords:Cyclamen persicum  tissue culture  rapid propagation  
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