| 正交试验法优选豆腐柴叶中果胶提取工艺 |
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| 引用本文： | 陈红玲,段红,翟科峰,曹稳根.正交试验法优选豆腐柴叶中果胶提取工艺[J].淮北煤炭师范学院学报(自然科学版),2011,32(3). |
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| 作者姓名： | 陈红玲 段红 翟科峰 曹稳根 |
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| 作者单位： | 宿州学院化学与生命科学院,安徽宿州,234000 |
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| 基金项目： | 安徽省教育厅自然科学研究项目(KJ2011B182); 宿州学院自然科学项目(2009yzk07);宿州学院生物化学与分析生物学重点学科项目(szxyzdxk200901) |
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| 摘 要： | 以豆腐柴叶为原料,采用酸水解、活性炭脱色和乙醇沉淀等方法提取其果胶物质.通过正交试验和方差分析确定果胶提取的关键操作过程.酸解的最佳操作条件为:酸解溶液pH 2.0、酸解时间60 min、酸解温度85℃、料液比1:8.在此条件下果胶的提取率为15.77%,此结果可为豆腐柴叶的开发利用提供技术依据.
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| 关 键 词： | 豆腐柴叶 果胶 酸解 提取工艺 |
Study on Extraction Technology of Pectin from Premna Micropheua Leaves with Orthogonal Design |
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| CHEN Hong-ling,DUAN Hong,ZHAI Ke-feng,CAO Wen-gen.Study on Extraction Technology of Pectin from Premna Micropheua Leaves with Orthogonal Design[J].Journal of Huaibei Coal Industry Teachers College(Natural Science edition),2011,32(3). |
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| Authors: | CHEN Hong-ling DUAN Hong ZHAI Ke-feng CAO Wen-gen |
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| Institution: | CHEN Hong-ling,DUAN Hong,ZHAI Ke-feng,CAO Wen-gen(Department of Chemistry &Life Science,Suzhou College,234000,Suzhou,Anhui,China) |
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| Abstract: | In this study,Premna Micropheua leaves were used as raw material to extract pectin.The extraction process of pectin from Premna Micropheua leaves with acid hydrolysis,activated carbon decolorizing,ultrafiltration concentration and ethanol precipitation were optimized via orthogonal test and variance analysis.The optimal extraction conditions are as follows：acid solution（pH=2） as extraction solvent,extraction time 60 minute,temperature 85 ℃and the ratio of material to solvent 1：8.Under these conditions,the extraction rate is up to 15.77%.The optimal process is stable and feasible. |
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| Keywords: | Premna Micropheua leaves pectin acid solution extraction technique |
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