| 横纹肌肉瘤多药耐药细胞系RD/VCR的建立 |
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| 引用本文： | 李玉皓,李胜芝,宋国祥,张新.横纹肌肉瘤多药耐药细胞系RD/VCR的建立[J].南开大学学报,2004,37(3):103-108. |
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| 作者姓名： | 李玉皓 李胜芝 宋国祥 张新 |
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| 作者单位： | [1]南开大学医学院微观形态学教研室，天津300071 [2]天津医科大学第二医院，天津300071 |
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| 基金项目： | 南开大学人事处科研启动基金资助项目 |
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| 摘 要： | 横纹肌肉瘤是一种高度恶性的软组织肿瘤，化疗是最主要的辅助治疗手段．然而，肿瘤细胞对药物产生多药耐药常常导致治疗失败．本实验以长春新碱为诱导剂，在体外成功建立了耐药细胞系DD／VCR，为进一步研究横纹肌肉瘤的耐药机理制作了模型．耐药前后细胞超微结构发生显变化，mdr1及mrp基因，P-糖蛋白及多药相关蛋白的表达与横纹肌肉瘤多药耐药的发生有关。
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| 关 键 词： | 横纹肌肉瘤 多药耐药 P—糖蛋白 多药耐药相关蛋白 |
| 文章编号： | 0465-7942(2004)03-0103-06 |
| 修稿时间： | 2003年7月14日 |
ESTABLISHMENT OF A MULTIDRUG RESISTANT RHABDOMYOSARCOMA CELL LINE RD/VCR |
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| LI Yuhao,LI Shengzhi,SONG Guoxiang,ZHANG Xin.ESTABLISHMENT OF A MULTIDRUG RESISTANT RHABDOMYOSARCOMA CELL LINE RD/VCR[J].Acta Scientiarum Naturalium University Nankaiensis,2004,37(3):103-108. |
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| Authors: | LI Yuhao LI Shengzhi SONG Guoxiang ZHANG Xin |
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| Abstract: | Rhabdomyosarcoma is a malignant soft tissue neoplasm. Chemotherapy constitutes the chief supplementary method in the treatment of rhabdomyosarcoma. However, the appearance of multidrug resistance (MDR) is a major obstacle to successful chemotherapy. In the present study, a multidrug resistant cell line cell line RD/VCR, which showed a typical resistant phenotype to anti-cancer drugs, was induced in vitro by vincristine (VCR). Morphological changes were found in the ultra structure. The expression of mdrl and mrp gene, P-glycoprotein (P-gp) and multidrug resistance associated protein (MRP) were detected by RT-PCR and immunohistochemistry respectively. The mechanism of MDR in rhabdomyosarcoma might be associated with the expression of these genes and proteins. |
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| Keywords: | Rhabdomyosarcoma Multidrug resistance P-glycoprotein (P-gp ) Multidrug resistance associated protein (MRP) |
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