细胞的增殖与凋亡在胎肺支气管上皮细胞发育过程的观察

目的：观察不同发育阶段人胎肺支气管粘膜上皮细胞的凋亡，以及增殖细胞核抗原（ＰＣＮＡ）的表达特征，并探讨细胞的凋亡与增殖在胎肺发育中的关系。方法：用原位核酸末端杂交，检测了１６～３２周人胎肺组织的凋亡细胞阳性表达率，用免疫细胞化学技术检测了同期人胎肺组织的ＰＣＮＡ阳性表达率。结果：人胎肺支气管粘膜上皮细胞在胎肺发育的早、中期（即假腺期和小管期），细胞的凋亡与增殖均明显较晚期旺盛，而且两者均于胎肺发育的第２４周达到最高蜂，以后细胞的凋亡与增殖活动减弱；但凋亡与增殖细胞在不同发育时期定位有不同。结论：细胞的凋亡与增殖行为在胎肺支气管上皮细胞发育分化的整个过程中普遍存在，分工协作，对于胎肺的形态发生及功能完善共同起作用。

Observation of Cell Proliferation and Apoptosis During Development of Bronchial in Fetal Lungs

Aim: examine the expressive characteristics of cell apoptosis and proliferating cell nuclear antigen(PCNA) in bronchial epithelial cells during the developmental stages.Methods: the positve ratio of apoptotic cells by use of in situ hybridization and the positive ratio of proliferative cell by use of immunocytochemistry techonique.Results: In the early and middle stages of the developmental fetal lung(the pseudoglandular and canalicular stages), cell apoptosis and proliferations of the bronchial epithelial were obviously more active than the late stage(the terminal sac stage),and both expressive rate reached the peak at the 24 th week;however,the positive reactions were respectively different at individual developmental stages and in different locations. Conclusion: the activities of cell apoptosis and proliferation were exsisted in the whole developmental process of brochial epithelial cells in fetal lung,and they cooperated harmoniously in order to establish a perfect shape and function of a mature lung.