| RP—HPLC测定青蒿中青蒿素的含量 |
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| 引用本文： | 黄海滨,奉建芳.RP—HPLC测定青蒿中青蒿素的含量[J].广西大学学报(自然科学版),1994,19(2):194-196. |
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| 作者姓名： | 黄海滨 奉建芳 |
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| 作者单位： | 广西中医学院药学系，湖南中医学院 |
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| 摘 要： | 采用反相高效液相色谱法，以对羟基苯甲酸乙酯为内标，测定了青蒿中青蒿素的合量。Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ柱，甲醇－醋酸盐缓冲液（０．０１ｍｏｌ／Ｌ，ｐＨ５．８）为流动相，ＵＶ２６０ｕｍ波长处检测。实验结果平均回收率为１０２．２％，相对标准偏差为１．０７％。方法快速、简便、准确。
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| 关 键 词： | 青蒿 青蒿素 高效液相色谱 |
RP HPLC Determination of Artemisininein Artemisia annua L. |
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| Huang Haibin,Cen Jiaming,Feng Jianfang.RP HPLC Determination of Artemisininein Artemisia annua L.[J].Journal of Guangxi University(Natural Science Edition),1994,19(2):194-196. |
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| Authors: | Huang Haibin Cen Jiaming Feng Jianfang |
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| Abstract: | he content of .Artemisinine in Artemisia annua was determined by RP--HPLC. Chromatographic conditions were f column, Shim-- pack ODS, mobile phase, methanol / acetate buffer(0.0l mol / L, PHS.8); internal standard, Ethyl p-hydroxybenzoate; detecated at UV 280um. The average recovery was 102.2% and RSD was 1 .07%. This method is quick, simple and accurate. |
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| Keywords: | artemisia annua artemisinine ethyl p-hydroxybenzoate HPLC |
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