苏云金芽孢杆菌在固态和液态基质中的生长差别研究

在同等条件下,采用同一培养基的固态和液态两种环境,对苏云金芽孢杆菌中国标准品(BtCS3ab-1991)的发酵质量进行了比较研究.结果表明:固态发酵培养的Bt芽孢平均长度0.028mm,液态发酵平均为0.017mm,含菌量在28h分别为0.88×1010个/l和0.54×1010个/l.发酵过程中菌体浓度在生长曲线上表现为固态发酵高于液态,缩短发酵时间2h,且接种量对固态发酵的影响也较小.而在虫害研究方面,取食含固态药液菜叶的小菜蛾羽化率较液体降低10%,死亡率差异显著.

Differences in growth and metabolism of Bacillus thuringiensis grown in the Solid-state and in the Fluid-state Fermentation

This paper reported the comparison of the effect of spores from solid medium and fluid medium with the Bacillus thuringiensis strain CS3ab-1991, on the same conditions. Results show that the average length of spores of solid-state fermentation is 0.028mm, while 0.017mm for that of fluid-state fermentation,0.88×1010/l and 0.54×1010/l Bt at 28h.The growth curve shows that cell concentrations of solid-state fermentation are much higher than it of fluid-state, solid-state fermentation shorten the period of 2h, and the inoculation number have a little effect on Bt of solid-state fermentation.The Plutella xylostella(L.)`s emergence rate of solid medium is 10% less than of that of fluid, and there is a significant difference of death rate.