| 液氮速冻法快速回收小分子DNA片段 |
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| 引用本文： | 王文锋,穆灵敏,王红霞,张光谋. 液氮速冻法快速回收小分子DNA片段[J]. 河南师范大学学报(自然科学版), 2009, 37(3) |
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| 作者姓名： | 王文锋 穆灵敏 王红霞 张光谋 |
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| 作者单位： | 新乡医学院,生命科学技术系,河南,新乡,453003;新乡医学院,形态学实验室,河南,新乡,453003 |
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| 基金项目： | 河南省科技厅2007年立项课题 |
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| 摘 要： | 介绍一种高效回收小分子DNA片段的方法,只需两个离心管及一块玻璃棉纸,凝胶速冻后简单离心即可回收DNA.实验证明这种回收DNA的方法具有简便快捷、成本低和效率高等优点,是一种切实可行的方法.
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| 关 键 词： | 小分子DNA 回收 液氮 速冻 |
A Fast Method for Recoverying Small Molecule DNA Fragment by Quick-Frozen in Liquid Nitrogen |
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| WANG Wen-feng,MU Ling-min,WANG Hong-xia,ZHANG Guang-mou. A Fast Method for Recoverying Small Molecule DNA Fragment by Quick-Frozen in Liquid Nitrogen[J]. Journal of Henan Normal University(Natural Science), 2009, 37(3) |
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| Authors: | WANG Wen-feng MU Ling-min WANG Hong-xia ZHANG Guang-mou |
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| Affiliation: | a.Department of Life Sciences and Technology;b.Lab of morphology;Xinxiang Medical College;Xinxiang 453003;China |
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| Abstract: | This article introduces an efficient way to recover small molecular fragments of DNA methods,just two centrifuge tubes and a piece of tissue paper glass.After the quick-freeze gel and a simple centrifuge,DNA can be recovered simply.The result of test proves that the recovery of the method is simple fast,low cost and high efficiency,which is a realistic and practical approach. |
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| Keywords: | small molecula DNA recovery liquid nitrogen quick-freeze |
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