结合适配体技术的磁性粒子表面蛋白分子印迹材料研制

本文将具有高度特异性的生物分子核酸适配体引入分子印迹技术，并结合磁性纳米粒子材料比表面积大、操作简便性和易于修饰性于一体，制备出了新型蛋白分子印迹材料.首先获得性能更好的硅烷化的磁性微球，之后加入功能单体、交联剂和衍生化适配体，以溶菌酶为模板分子，制备出磁性溶菌酶蛋白分子印迹适配体-纳米粒子.实验结果显示，不同材料的蛋白吸附能力顺序由高到低分别为结合适配体的蛋白印迹材料，结合适配体的非蛋白印记材料，蛋白印迹材料和无适配体的非蛋白分子印记材料.初步研究表明，适配体与磁性粒子的结合可以有效提高蛋白质分子印迹材料的印迹效果.

Preparation of Surface Protein Imprinting Nanoparticles with Aptamers

In this paper,a highly specific biomolecule,the nucleic acid aptamer,was introduced into the molecular imprinting technique.A new type of molecular imprinted material was prepared by combining the high specific surface area of the magnetic nanoparticle material,the ease of operation and the ease of modification.First the silicified magnetic microspheres with better performance were obtained,and then the functional monomers,crosslinking agents and derivatized aptamers were added.The lysozyme was used as the template molecule to prepare the magnetic lysozyme molecular imprinting aptamers-nanoparticles.The results showed that the protein adsorption capacity of different materials was,from high to low respectively,the protein imprinting material with aptamer,non-protein imprinting material with aptamer,protein imprinting material and nonimprinting material.The preliminary study shows that the introduction of the aptamer to the magnetic particles can effectively improve performance of the molecularly imprinted material.