不同寄主尖孢镰刀菌同工酶异质性的研究

利用聚丙烯酰胺凝胶电泳测定了来自不同寄主枯萎病原菌尖孢镰刀菌过氧化物酶同工酶(POD)和酯酶同工酶(EST).结果表明,过氧化物酶同工酶共有3条主酶带,其迁移率分别为:Rf=0.08,Rf=0.20和Rf=0.4.但不同寄主的尖孢镰刀菌POD同工酶存在着明显的差别,来源于花生的尖孢镰刀菌只有2条POD同工酶酶带,其迁移率为:Rf=0.20和Rf=0.08;来源于甜瓜的尖孢镰刀菌只有1条POD同工酶酶带,其迁移率为:Rf=0.20;来源于不同寄主的镰刀菌的EST同工酶谱之间也存在着较大的差别.来源于黄瓜、甜瓜和西瓜的镰刀菌的EST酶谱较为相似,都具有Rf=0.018,Rf=0.114和Rf=1.000的3条酶带,其它寄主来源的镰刀菌的EST酶谱间互相则存在着很大的差别.但这些菌株基本上都存在着Rf为0.018和1.000的2条酯酶同工酶酶带.

A Study on POD and EST Isozyme of Different Strains of Fusarium oxysporum from Different Crops

The peroxidase (POD) and esterase (EST) isozyme of different Fusarium oxysporum from different crops were analyzed by polyarcylamide gel eletrophoresis.The results showed that there were 3 main POD isozme bands;the relative migration rates were 0.08,0.24,and 0.4.The strain from the earthnut had only 2 POD isozyme bands and their relative migration rates were 0.20 and 0.08.The strain from melon had only 1 POD isozyme bands and its relative migration rate was 0.20.There were many differences found in esterase isozyme of the different strains of F.oxysporum,in whixh the main isozyme bands were similar in cucumber,melon and watermelon and their relative migration rates were 0.018,0.114,1.000,and that of the F.oxysporum from the other crops was much different.But they all had the main EST isozme bands whose relative migration rates were 0.018 and 1.000.