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墨西哥香荚兰染色体及其变异的研究初报
引用本文:陈小兰,邓攀博,杨明挚,周恒苍,陈善娜.墨西哥香荚兰染色体及其变异的研究初报[J].云南大学学报(自然科学版),2004,26(4):360-363.
作者姓名:陈小兰  邓攀博  杨明挚  周恒苍  陈善娜
作者单位:1. 云南大学,生物技术系,云南,昆明,650091
2. 云南省香荚兰公司,云南,昆明,650091
基金项目:云南省科技"九五"攻关项目(95A2-8),"211工程"二期建设费及植物学博士点经费资助.
摘    要: 对栽培品种墨西哥香荚兰(Vanilla planifolia Andr.)进行了常规染色体分析,并对其杂交品种、无性繁殖植株(组培苗、扦插苗)和野生品种滇南香荚兰(V.siamensis Rolfeex Downie)的染色体变异进行了观察比较.结果表明,香荚兰染色体的大小在0.2~2μm之间,为小染色体,2N=28,经长期继代培养的组培苗染色体出现变异的频率明显超过扦插苗和野生品种,微核、染色体桥和染色体断片的频率明显增高.

关 键 词:墨西哥香荚兰  滇南香荚兰  染色体变异  微核  染色体桥  染色体断片
文章编号:0258-7971(2004)04-0360-04
修稿时间:2003年12月17

A primary study on chromosome and its variation of Vanilla planifolia Andr.
CHEN Xiao-lan,DENG Pan-bo,YANG Ming-zhi,ZHOU Heng-cang,CHEN Shan-na.A primary study on chromosome and its variation of Vanilla planifolia Andr.[J].Journal of Yunnan University(Natural Sciences),2004,26(4):360-363.
Authors:CHEN Xiao-lan  DENG Pan-bo  YANG Ming-zhi  ZHOU Heng-cang  CHEN Shan-na
Institution:CHEN Xiao-lan~1,DENG Pan-bo~1,YANG Ming-zhi~1,ZHOU Heng-cang~2,CHEN Shan-na~1
Abstract:Chromosome of Vanilla planifolia Andr.were studied in this paper.Chromosome abnormity of asexual reproduction (including tissue culture and cuttage)plants of Vanilla planifoliar were observed and compared with its hybrid breed and wild breed Vanill siamensis Rolfe ex Downie.The results showed that V.planifolia have 28 small chromosomes whose size ranges from 0.2 to 2 micron.The frequency of chromosome abnormity of tissue culture plants was higher than that of cuttage and wild plants.It's frequency of micronuclei,chromosome bridge and chromosome lag apparently increased.
Keywords:Vanilla planifolia Andr    Vanill siamensis Rolfe ex Downiewere    chromosome abnormity micronuclei  chromosome bridge  chromosome lag
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