| 多通道电化学基因传感阵列用于同时检测AML相关多药耐药基因的新方法研究 |
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| 引用本文： | 王昆,张书仪,颜志文,魏吟秋,李碧艳,陈方,陈庆.多通道电化学基因传感阵列用于同时检测AML相关多药耐药基因的新方法研究[J].福州大学学报(自然科学版),2020,48(5). |
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| 作者姓名： | 王昆 张书仪 颜志文 魏吟秋 李碧艳 陈方 陈庆 |
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| 作者单位： | 厦门大学附属第一医院,厦门大学药学院,厦门大学附属第一医院,厦门大学附属第一医院,厦门大学附属第一医院,厦门大学附属第一医院,厦门大学药学院 |
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| 基金项目： | 国家自然科学基金资助项目（81903585）；福建省卫生计生中青年骨干人才培养项目（2017-ZQN-85）；厦门市科技计划（科技惠民）项目（3502Z20164001） |
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| 摘 要： | 本研究筛选并制备高特异性 DNA 探针,与纳米粒子标记技术相结合,设计多通道电化学基因传感阵列检测模式,建立灵敏、快速、简便、经济的AML相关多药耐药基因(MDR1和MRP)检测新方法。该方法具有较好的特异性、灵敏度和重现性,能够在1.0×10-14 ~ 1.0×10-12 M和5.0×10-14 ~ 5.0×10-12 M范围内,分别实现对AML 相关多药耐药基因序列MDR1和MRP的定量检测。该方法有望为预测肿瘤治疗效果和临床制定治疗方案提供参考依据。
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| 关 键 词： | 多通道电化学传感阵列 急性髓系白血病 多药耐药基因 |
| 收稿时间： | 2019/10/23 0:00:00 |
| 修稿时间： | 2019/11/12 0:00:00 |
A novel method about multichannel electrochemical gene sensor array for simultaneous detection of AML multidrug resistance genes |
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| WANG Kun,ZHANG Shuyi,YAN Zhiwen,WEI Yinqiu,LI Biyan,CHEN Fang and CHEN Qing.A novel method about multichannel electrochemical gene sensor array for simultaneous detection of AML multidrug resistance genes[J].Journal of Fuzhou University(Natural Science Edition),2020,48(5). |
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| Authors: | WANG Kun ZHANG Shuyi YAN Zhiwen WEI Yinqiu LI Biyan CHEN Fang and CHEN Qing |
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| Institution: | Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian,Faculty of Pharmacy,Xiamen University,Xiamen,Fujian,Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian,Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian,Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian,Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian,Faculty of Pharmacy,Xiamen University,Xiamen,Fujian |
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| Abstract: | In the study, the high specific DNA probes were screened and prepared, and a multichannel electrochemical gene sensor array mode was designed combining with nanoparticle labeling technology. A sensitive, fast, simple and economic new method was established for simultaneous detection of AML related multidrug resistance genes (MDR1 and MRP). The method showed good specificity, sensitivity and repeatability. The method can realize quantitative detection for MDR1 from 1.0×10-14 to 1.0×10-12 M and MRP from 5.0×10-14 to 5.0×10-12 M , respectively. The method is expected to provide a reference for predicting the treatments of cancer and making clinical treatment strategies. |
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| Keywords: | multichannel electrochemical gene sensor array acute myeloid leukemia multidrug resistance genes |
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